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Whole cell based mi...
Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli
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- Idil, Neslihan (författare)
- Hacettepe University,Lund University
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- Hedström, Martin (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze HB
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- Denizli, Adil (författare)
- Hacettepe University
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- Mattiasson, Bo (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,CapSenze HB
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- Engelska 9 s.
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Ingår i: Biosensors and Bioelectronics. - : Elsevier BV. - 0956-5663. ; 87, s. 807-815
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×102–1.0×107 CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Miljöbioteknik -- Diagnostisk bioteknologi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Biotechnology -- Diagnostic Biotechnology (hsv//eng)
Nyckelord
- Capacitive biosensor
- Escherichia coli
- Microcontact imprinting
- N-methacryloyl-L-histidine methylester
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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