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Effects of various ...
Effects of various neuropeptide Y/peptide YY fragments on electrically-evoked contractions of the rat vas deferens
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- Grundemar, L (författare)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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- Håkanson, R (författare)
- Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2012-07-19
- 1990
- Engelska.
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Ingår i: British Journal of Pharmacology. - : Wiley. - 0007-1188. ; 100:1, s. 190-192
- Relaterad länk:
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http://dx.doi.org/10...
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https://europepmc.or...
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Abstract
Ämnesord
Stäng
- 1. The effects of various neuropeptide Y (NPY) and peptide YY (PYY) fragments on electrically-evoked twitches in the rat isolated vas deferens were studied and compared with the effects of full length NPY and PYY. The aim was to identify the shortest NPY/PYY fragments that are capable of suppressing the contractions. 2. NPY (1-36) and C-terminal fragments of NPY (from 11-36 to 22-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. On the whole there seemed to be a gradual lowering of the pIC50 values with progressive shortening of the NPY fragments (except for fragments 16-36 and 22-36 that had rather high pIC50 values). NPY 23-36, 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). NPY 26-36 was without effect as were C-terminal carboxy-deaminated NPY and glycine extended NPY (NPY-Gly-Lys-Arg). 3. PYY (1-36) and C-terminal fragments of PYY (from 11-36 to 23-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. PYY 1-36 was more potent than any of the fragments. There was a tendency for shorter fragments to have lower pIC50 values. PYY 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). PYY 26-36 was without effect. 4. The findings suggest that the 12 C-terminal amino acid residues of NPY and PYY are the minimum length required to activate the Y2-receptor.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Amino Acid Sequence
- Animals
- Electric Stimulation
- In Vitro Techniques
- Male
- Molecular Sequence Data
- Muscle Contraction/drug effects
- Muscle, Smooth/drug effects
- Neuropeptide Y/pharmacology
- Peptide Fragments/pharmacology
- Peptide YY
- Peptides/pharmacology
- Rats
- Rats, Inbred Strains
- Swine
- Vas Deferens/drug effects
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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