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Endoplasmic reticul...
Endoplasmic reticulum stress associated with caspases-4 and-2 mediates korbazol-induced B-chronic lymphocytic leukemia cell apoptosis
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Popovic, S. (författare)
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Baskic, D. (författare)
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Djurdjevic, P. (författare)
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visa fler...
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Zelen, I. (författare)
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Mitrovic, M. (författare)
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Nikolic, I. (författare)
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Avramovic, D. (författare)
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- Radenkovic, Miljana (författare)
- Lund University,Lunds universitet,Cellpatologi, Malmö,Forskargrupper vid Lunds universitet,Cell Pathology, Malmö,Lund University Research Groups
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Arsenijevic, N. (författare)
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visa färre...
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(creator_code:org_t)
- 2010
- 2010
- Engelska.
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Ingår i: Journal of Buon. - 1107-0625. ; 15:4, s. 783-790
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
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- Purpose: B-cell chronic lymphocytic leukemia (B-CLL) is an incurable disease that rapidly develops drug resistance. Therefore there is a need for identifying new agents that will improve the therapeutic outcome. Korbazol is a natural product known to exert cytotoxic effect on the in vitro survival of leukemic cells. The aim of this study was to investigate the mechanism of korbazol-induced apoptosis in B-CLL leukemic cells. Methods: Peripheral blood mononuclear cells from 10 B-CLL patients were used for assessing the effect of caspase inhibitors and chelator of intracellular Ca2+ Results: Cell death rate induced by the tested compound was decreased with the caspase-3 inhibitor Ac-DEVD-CHO, and the inhibitors of caspase-2 (Z-VDVAD-FMK) and -4 (ZYVAD-FMK), but not with the caspase-9 inhibitor z-LEHD-FMK and caspase-8 inhibitor z-IETD-FMK No significant release of cytochrome C (cyt C) from mitochondria to the cytosol of B-CLL cells treated with korbazol was observed. Moreover, chelating of intracellular Ca2+ with BAPTA-AM almost completely abolished the cytotoxic effect of korbazol. Conclusion: Engagement of caspases-2 and -4 and mobilization of intracellular Ca2+ indicate involvement of endoplasmic reticulum (ER) stress in apoptosis induced by korbazol.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- korbazol
- endoplasmic reticulum stress
- chronic lymphocytic leukemia
- apoptosis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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