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Model system for st...
Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere.
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- Odham, G. (författare)
- Lund University
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- Tunlid, A. (författare)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science
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- Valeur, A. (författare)
- Lund University
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visa fler...
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- Sundin, P. (författare)
- Lund University
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- White, D. C. (författare)
- Florida State University
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visa färre...
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(creator_code:org_t)
- 1986
- 1986
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 52:1, s. 191-196
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a composition resembling that of natural rape root exudate was pumped through the membrane at 0.5 ml/h. Scanning electron microscopic examinations indicated that the inoculum formed microcolonies embedded in exopolymers evenly distributed over the membrane surface. The lipid composition and content of poly-beta-hydroxybutyrate in free-living and adhered cells were determined by gas chromatography. The bacterial consumption of amino acids in the exudate was also studied.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
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- ref (ämneskategori)
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