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Affinity purificati...
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Nilsson, Rickard OLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine
(författare)
Affinity purification of human factor h on polypeptides derived from streptococcal m protein: enrichment of the y402 variant.
- Artikel/kapitelEngelska2013
Förlag, utgivningsår, omfång ...
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2013-11-21
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Public Library of Science (PLoS),2013
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electronicrdacarrier
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LIBRIS-ID:oai:lup.lub.lu.se:87477640-3236-4abe-89f7-415e64614351
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https://lup.lub.lu.se/record/4178900URI
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https://doi.org/10.1371/journal.pone.0081303DOI
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Språk:engelska
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Sammanfattning på:engelska
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Recent studies indicate that defective activity of complement factor H (FH) is associated with several human diseases, suggesting that pure FH may be used for therapy. Here, we describe a simple method to isolate human FH, based on the specific interaction between FH and the hypervariable region (HVR) of certain Streptococcus pyogenes M proteins. Special interest was focused on the FH polymorphism Y402H, which is associated with the common eye disease age-related macular degeneration (AMD) and has also been implicated in the binding to M protein. Using a fusion protein containing two copies of the M5-HVR, we found that the Y402 and H402 variants of FH could be efficiently purified by single-step affinity chromatography from human serum containing the corresponding protein. Different M proteins vary in their binding properties, and the M6 and M5 proteins, but not the M18 protein, showed selective binding of the FH Y402 variant. Accordingly, chromatography on a fusion protein derived from the M6-HVR allowed enrichment of the Y402 protein from serum containing both variants. Thus, the exquisite binding specificity of a bacterial protein can be exploited to develop a simple and robust procedure to purify FH and to enrich for the FH variant that protects against AMD.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Lannergård, JonasLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-jlg
(författare)
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Morgan, B Paul
(författare)
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Lindahl, GunnarLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-gli
(författare)
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Gustafsson, MattiasLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mig-mgu
(författare)
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Avdelningen för medicinsk mikrobiologiInstitutionen för laboratoriemedicin
(creator_code:org_t)
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Ingår i:PLoS ONE: Public Library of Science (PLoS)8:111932-6203
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