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A hemolytic assay f...
A hemolytic assay for the estimation of functional mannose-binding lectin levels in human serum
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Kuipers, S (författare)
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Aerts, PC (författare)
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- Sjöholm, Anders (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine
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Harmsen, T (författare)
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van Dijk, H (författare)
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(creator_code:org_t)
- 2002
- 2002
- Engelska.
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Ingår i: Journal of Immunological Methods. - 1872-7905. ; 268:2, s. 149-157
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A simple assay was developed to estimate functional mannose-binding lectin (MBL) levels in serum based on the principle of yeast-induced bystander lysis of chicken erythrocytes (ChE). The assay is sensitive to inhibition by ethylene glycol bis-(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) (which allows alternative pathway activation), ethylene diamine tetraacetic acid (EDTA), mannose, N-acetylglucosamine and C1 esterase inhibitor (C1-INH), whereas it was not inhibited by galactose. A high-titer human anti-mannan antibody-containing serum with 0.06 mug MBL/ml gave a functional signal corresponding to 0.12 mug equivalents MBL/ml, indicating that anti-mannan antibodies are poorly hemolytic in the assay. The assay is well suited for the large-scale testing of patient samples for a functional MBL pathway of complement activation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- bystander hemolysis
- functional assay
- MBL
- lectin pathway
- Saccharomyces cerevisiae
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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