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Sökning: onr:"swepub:oai:lup.lub.lu.se:9f5d611e-ae2d-4f31-9960-ae1b81b0a1d0" > Clustering of infra...

  • Kobrina, Yevgeniya (författare)

Clustering of infrared spectra reveals histological zones in intact articular cartilage

  • Artikel/kapitelEngelska2012

Förlag, utgivningsår, omfång ...

  • Elsevier BV,2012

Nummerbeteckningar

  • LIBRIS-ID:oai:lup.lub.lu.se:9f5d611e-ae2d-4f31-9960-ae1b81b0a1d0
  • https://lup.lub.lu.se/record/2563298URI
  • https://doi.org/10.1016/j.joca.2012.01.014DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • Objective: Articular cartilage (AC) exhibits specific zonal structure that follows the organization of collagen network and concentration of tissue constituents. The aim of this study was to investigate the potential of unsupervised clustering analysis applied to Fourier transform infrared (FIR) microspectroscopy to detect depth-dependent structural and compositional differences in intact AC. Method: Seven rabbit and eight bovine intact patellae AC samples were imaged using FTIR microspectroscopy and normalized raw spectra were clustered using the fuzzy C-means algorithm. Differences in mean spectra of clusters were investigated by quantitative estimation of collagen and proteoglycan (PG) contents, as well as by careful visual investigation of locations of spectral changes. Results: Clustering revealed the typical layered structure of AC in both species. However, more distinct clusters were found for rabbit samples, whereas bovine AC showed more complex layered structure. In both species, clustering structure corresponded with that in polarized light microscopic (PLM) images; however, some differences were also observed. Spectral differences between clusters were identified at the same spectral locations for both species. Estimated PG/collagen ratio decreased significantly from superficial to middle or deep zones, which might explain the difference in clustering results compared to PLM. Conclusion: FTIR microspectroscopy in combination with cluster analysis allows detailed examination of spatial changes in AC. As far as we know, no previous single technique could reveal a layered structure of AC without any a priori information. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Rieppo, Lassi (författare)
  • Saarakkala, Simo (författare)
  • Jurvelin, Jukka S. (författare)
  • Isaksson, HannaLund University,Lunds universitet,Hållfasthetslära,Institutionen för byggvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Solid Mechanics,Department of Construction Sciences,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)soli-hai (författare)
  • HållfasthetsläraInstitutionen för byggvetenskaper (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Osteoarthritis and Cartilage: Elsevier BV20:5, s. 460-4681063-4584

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