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Quantifying the rat...
Quantifying the rate, degree, and heterogeneity of morphological change during an epithelial to mesenchymal transition using digital holographic cytometry
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- Kamlund, Sofia (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science,Phase Holographic Imaging PHI AB
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- Janicke, Birgit (författare)
- Phase Holographic Imaging PHI AB
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- Alm, Kersti (författare)
- Phase Holographic Imaging PHI AB
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- Judson-Torres, Robert L. (författare)
- University of Utah
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- Oredsson, Stina (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Functional zoology,Department of Biology,Faculty of Science,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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(creator_code:org_t)
- 2020-07-09
- 2020
- Engelska.
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Ingår i: Applied Sciences (Switzerland). - : MDPI AG. - 2076-3417. ; 10:14
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://www.mdpi.com...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Cells in complex organisms can transition between epithelial and mesenchymal phenotypes during both normal and malignant physiological events. These two phenotypes are not binary, but rather describe a spectrum of cell states along an axis. Mammalian cells can undergo dynamic and heterogenous bidirectional interconversions along the epithelial-mesenchymal phenotypic (EMP) spectrum, and such transitions are marked by morphological change. Here, we exploit digital holographic cytometry (DHC) to develop a tractable method for monitoring the degree, kinetics, and heterogeneity of epithelial and mesenchymal phenotypes in adherent mammalian cell populations. First, we demonstrate that the epithelial and mesenchymal states of the same cell line present distinct DHC-derived morphological features. Second, we identify quantitative changes in these features that occur hours after induction of the epithelial to mesenchymal transition (EMT). We apply this approach to achieve label-free tracking of the degree and the rate of EMP transitions. We conclude that DHC is an efficient method to investigate morphological changes during transitions between epithelial and mesenchymal states.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Digital holographic cytometry
- Epithelial tomesenchymal transition
- Quantitative phase imaging
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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