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Novel xylan-binding...
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Cicortas Gunnarsson, LaviniaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Department of Immunotechnology, Lund University, Lund, Sweden
(författare)
Novel xylan-binding properties of an engineered family 4 carbohydrate-binding module
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:a3e7378d-88ce-480e-a555-8263d68f47fc
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https://lup.lub.lu.se/record/689640URI
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https://doi.org/10.1042/BJ20070128DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-64488URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Funding Agency:Biotechnology and Biological Sciences Research Council BB/C005074/1
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Molecular engineering of ligand-binding proteins is commonly used for identification of variants that display novel specificities. Using this approach to introduce novel specificities into CBMs (carbohydrate-binding modules) has not been extensively explored. Here, we report the engineering of a CBM, CBM42 from the Rhodothermits marinus xylanase Xyn10A, and the identification of the X-2 variant. As compared with the wildtype protein, this engineered module displays higher specificity for the polysaccharide xylan, and a lower preference for binding xylo-oligomers rather than binding the natural decorated polysaccharide. The mode of binding of X-2 differs from other xylan-specific CBMs in that it only has one aromatic residue in the binding site that can make hydrophobic interactions with the sugar rings of the ligand. The evolution of CBM4-2 has thus generated a xylan-binding module with different binding properties to those displayed by CBMs available in Nature.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Montanier, CedricInstitute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
(författare)
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Tunnicliffe, Richard B.Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK
(författare)
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Williamson, Mike R.
(författare)
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Gilbert, Harry J.Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
(författare)
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Nordberg Karlsson, EvaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Department of Biotechnology, Lund University, Lund, Sweden(Swepub:lu)biot-eno
(författare)
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Ohlin, MatsLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Department of Immunotechnology, Lund University, Lund, Sweden(Swepub:lu)immt-moh
(författare)
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Williamson, Mike PDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK
(författare)
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Institutionen för immunteknologiInstitutioner vid LTH
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemical Journal: Portland Press406, s. 209-2140264-60211470-8728
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