Sökning: onr:"swepub:oai:lup.lub.lu.se:a6fe995c-14fb-477f-91b1-d615262bebc3" >
Actin retrograde fl...
Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions
-
- Swaminathan, Vinay (författare)
- National Heart Lung and Blood Institute,Marine Biological Laboratory
-
- Kalappurakkal, Joseph Mathew (författare)
- National Centre for Biological Sciences,Marine Biological Laboratory
-
- Mehta, Shalin B. (författare)
- Marine Biological Laboratory
-
visa fler...
-
- Nordenfelt, Pontus (författare)
- Lund University,Lunds universitet,epIgG,Forskargrupper vid Lunds universitet,Lund University Research Groups,Harvard University,Boston Children's Hospital,Marine Biological Laboratory
-
- Moore, Travis I. (författare)
- Boston Children's Hospital,Harvard University,Marine Biological Laboratory
-
- Koga, Nobuyasu (författare)
- Institute for Molecular Science,University of Washington
-
- Baker, David A. (författare)
- University of Washington
-
- Oldenbourg, Rudolf (författare)
- Marine Biological Laboratory
-
- Tani, Tomomi (författare)
- Marine Biological Laboratory
-
- Mayor, Satyajit (författare)
- Marine Biological Laboratory,National Centre for Biological Sciences
-
- Springer, Timothy A. (författare)
- Marine Biological Laboratory,Boston Children's Hospital,Harvard University
-
- Waterman, Clare M. (författare)
- Marine Biological Laboratory,National Heart Lung and Blood Institute
-
visa färre...
-
(creator_code:org_t)
- 2017-10-03
- 2017
- Engelska 6 s.
-
Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 114:40, s. 10648-10653
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://www.pnas.org...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Integrins are transmembrane receptors that, upon activation, bind extracellular ligands and link them to the actin filament (F-actin) cytoskeleton to mediate cell adhesion and migration. Cytoskeletal forces in migrating cells generated by polymerization- or contractility-driven “retrograde flow” of F-actin from the cell leading edge have been hypothesized to mediate integrin activation for ligand binding. This predicts that these forces should align and orient activated, ligand-bound integrins at the leading edge. Here, polarization-sensitive fluorescence microscopy of GFP-αVβ3 integrins in fibroblasts shows that integrins are coaligned in a specific orientation within focal adhesions (FAs) in a manner dependent on binding immobilized ligand and a talin-mediated linkage to the F-actin cytoskeleton. These findings, together with Rosetta modeling, suggest that integrins in FA are coaligned and may be highly tilted by cytoskeletal forces. Thus, the F-actin cytoskeleton sculpts an anisotropic molecular scaffold in FAs, and this feature may underlie the ability of migrating cells to sense directional extracellular cues.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Cell migration
- Fluorescence polarization microscopy
- Mechanosensing
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Swaminathan, Vin ...
-
Kalappurakkal, J ...
-
Mehta, Shalin B.
-
Nordenfelt, Pont ...
-
Moore, Travis I.
-
Koga, Nobuyasu
-
visa fler...
-
Baker, David A.
-
Oldenbourg, Rudo ...
-
Tani, Tomomi
-
Mayor, Satyajit
-
Springer, Timoth ...
-
Waterman, Clare ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Cellbiologi
- Artiklar i publikationen
-
Proceedings of t ...
-
Proceedings of t ...
- Av lärosätet
-
Lunds universitet