Sökning: onr:"swepub:oai:lup.lub.lu.se:ac6091f2-28d4-43fd-acbb-90ff516b756d" >
Relationship betwee...
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics
-
Specht, Ina O. (författare)
-
Spano, Marcello (författare)
-
Hougaard, Karin S. (författare)
-
visa fler...
-
Manicardi, Gian C. (författare)
-
Bizzaro, Davide (författare)
-
Toft, Gunnar (författare)
-
- Giwercman, Aleksander (författare)
- Lund University,Lunds universitet,Reproduktionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Reproductive medicine, Malmö,Lund University Research Groups
-
Bonde, Jens-Peter E. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2012-10-15
- 2012
- Engelska.
-
Ingår i: Asian Journal of Andrology. - : Medknow. - 1008-682X .- 1745-7262. ; 14:6, s. 890-896
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
http://www.asiaandro...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P<0.01), more motile sperms (P=0.04) and fewer sperm head defects (P=0.05). These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone (FSH) and sexual hormone-binding globulin (SHBG) were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers. Asian Journal of Andrology (2012) 14, 890-896; doi: 10.1038/aja.2012.76; published online 15 October 2012
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Nyckelord
- apoptosis
- Bcl-x protein
- Fas-associated death domain protein
- fertility
- sperm chromatin structure assay (SCSA)
- spermatozoa
- TUNEL
- assay
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas