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Sökning: onr:"swepub:oai:lup.lub.lu.se:affef12b-cad5-4d6e-9d43-0ecbeb684d7a" > Actin filament guid...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005098naa a2200409 4500
001oai:lup.lub.lu.se:affef12b-cad5-4d6e-9d43-0ecbeb684d7a
003SwePub
008160401s2006 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/3983442 URI
0247 a https://doi.org/10.1021/la060854i2 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Sundberg, Mark4 aut
2451 0a Actin filament guidance on a chip: Toward high-throughput assays and lab-on-a-chip applications
264 c 2006-07-22
264 1b American Chemical Society (ACS),c 2006
520 a Biological molecular motors that are constrained so that function is effectively limited to predefined nanosized tracks may be used as molecular shuttles in nanotechnological applications. For these applications and in high-throughput functional assays (e. g., drug screening), it is important that the motors propel their cytoskeletal filaments unidirectionally along the tracks with a minimal number of escape events. We here analyze the requirements for achieving this for actin filaments that are propelled by myosin II motor fragments (heavy meromyosin; HMM). First, we tested the guidance of HMM-propelled actin filaments along chemically defined borders. Here, trimethylchlorosilane (TMCS)-derivatized areas with high-quality HMM function were surrounded by SiO2 domains where HMM did not bind actin. Guidance along the TMCS-SiO2 border was almost 100% for filament approach angles between 0 and 20 degrees but only about 10% at approach angles near 90 degrees. A model (Clemmens, J.; Hess, H.; Lipscomb, R.; Hanein, Y.; Bohringer, K. F.; Matzke, C. M.; Bachand, G. D.; Bunker, B. C.; Vogel, V. Langmuir 2003, 19, 10967-10974) accounted for essential aspects of the data and also correctly predicted a more efficient guidance of actin filaments than previously shown for kinesin- propelled microtubules. Despite the efficient guidance at low approach angles, nanosized (< 700 nm wide) TMCS tracks surrounded by SiO2 were not effective in guiding actin filaments. Neither was there complete guidance along nanosized tracks that were surrounded by topographical barriers (walls and roof partially covering the track) unless there was also chemically based selectivity between the tracks and surroundings. In the latter case, with dually defined tracks, there was close to 100% guidance. A combined experimental and theoretical analysis, using tracks of the latter type, suggested that a track width of less than about 200-300 nm is sufficient at a high HMM surface density to achieve unidirectional sliding of actin filaments. In accord with these results, we demonstrate the long- term trapping of actin filaments on a closed-loop track (width < 250 nm). The results are discussed in relation to lab-on-a-chip applications and nanotechnology-assisted assays of actomyosin function.
650 7a NATURVETENSKAPx Fysikx Den kondenserade materiens fysik0 (SwePub)103042 hsv//swe
650 7a NATURAL SCIENCESx Physical Sciencesx Condensed Matter Physics0 (SwePub)103042 hsv//eng
7001 a Bunk, Richardu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-rbu
7001 a Albet-Torres, Nuria4 aut
7001 a Kvennefors, Andersu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-ark
7001 a Persson, Fredrik4 aut
7001 a Montelius, Larsu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-lmo
7001 a Nicholls, Ian A.4 aut
7001 a Ghatnekar-Nilsson, Sarau Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)insek-sn
7001 a Omling, Päru Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-pom
7001 a Tagerud, Sven4 aut
7001 a Mansson, Alf4 aut
7102 a Fasta tillståndets fysikb Fysiska institutionen4 org
7730 t Langmuird : American Chemical Society (ACS)g 22:17, s. 7286-7295q 22:17<7286-7295x 0743-7463x 1520-5827
8564 u http://dx.doi.org/10.1021/la060854iy FULLTEXT
8564 8u https://lup.lub.lu.se/record/398344
8564 8u https://doi.org/10.1021/la060854i

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