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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04038naa a2200433 4500 | |
| 001 | oai:lup.lub.lu.se:b0c0e0f1-4642-49c6-b2f6-5e90eef64e4f | |
| 003 | SwePub | |
| 008 | 180323s2018 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:138145662 | |
| 024 | 7 | a https://lup.lub.lu.se/record/b0c0e0f1-4642-49c6-b2f6-5e90eef64e4f2 URI |
| 024 | 7 | a https://doi.org/10.1016/j.abb.2018.03.0082 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1381456622 URI |
| 040 | a (SwePub)lud (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Koruza, K.u Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science4 aut0 (Swepub:lu)biol-kak |
| 245 | 1 0 | a Deuteration of human carbonic anhydrase for neutron crystallography : Cell culture media, protein thermostability, and crystallization behavior |
| 264 | 1 | b Elsevier BV,c 2018 |
| 300 | a 8 s. | |
| 520 | a Deuterated proteins and other bio-derived molecules are important for NMR spectroscopy, neutron reflectometry, small angle neutron scattering, and neutron protein crystallography. In the current study we optimized expression media and cell culture conditions to produce high levels of 3 different deuterated human carbonic anhydrases (hCAs). The labeled hCAs were then characterized and tested for deuterium incorporation by mass spectrometry, temperature stability, and propensity to crystallize. The results show that is possible to get very good yields (>10 mg of pure protein per liter of cell culture under deuterated conditions) and that protein solubility is unaffected at the crystallization concentrations tested. Using unlabeled carbon source and recycled heavy water, we were able to get 65–77% deuterium incorporation, sufficient for most neutron-based techniques, and in a very cost-effective way. For most deuterated proteins characterized in the literature, the solubility and thermal stability is reduced. The data reported here is consistent with these observations and it was clear that there are measurable differences between hydrogenous and deuterated versions of the same protein in Tm and how they crystallize. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a Carbonic anhydrase | |
| 653 | a Deuteration | |
| 653 | a Minimal media | |
| 653 | a Neutron scattering | |
| 653 | a Thermal stability | |
| 700 | 1 | a Lafumat, B.u Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science4 aut0 (Swepub:lu)be0033la |
| 700 | 1 | a Knecht, W.u Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science4 aut0 (Swepub:lu)biol-wnk |
| 700 | 1 | a Fisher, S. Z.u Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,European Spallation Source ESS AB,Stiftelser och övriga anknutna verksamheter,Molecular Cell Biology,Department of Biology,Faculty of Science,Other Institutions and Utilities4 aut0 (Swepub:lu)biol-zfh |
| 710 | 2 | a Molekylär cellbiologib Biologiska institutionen4 org |
| 773 | 0 | t Archives of Biochemistry and Biophysicsd : Elsevier BVg 645, s. 26-33q 645<26-33x 0003-9861x 1096-0384 |
| 856 | 4 | u http://dx.doi.org/10.1016/j.abb.2018.03.008y FULLTEXT |
| 856 | 4 | u https://doi.org/10.1016/j.abb.2018.03.008 |
| 856 | 4 8 | u https://lup.lub.lu.se/record/b0c0e0f1-4642-49c6-b2f6-5e90eef64e4f |
| 856 | 4 8 | u https://doi.org/10.1016/j.abb.2018.03.008 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:138145662 |
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