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Long-term osteogeni...
Long-term osteogenic differentiation of human bone marrow stromal cells in simulated microgravity : novel proteins sighted
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- Montagna, Giulia (författare)
- University of Pavia
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- Pani, Giuseppe (författare)
- University of Milan
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- Flinkman, Dani (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,University of Turku
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- Cristofaro, Francesco (författare)
- University of Pavia
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- Pascucci, Barbara (författare)
- CNR Istituto per le Tecnologie Applicate ai Beni Culturali (CNR-ITABC)
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- Massimino, Luca (författare)
- San Raffaele Hospital
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- Lamparelli, Luigi Antonio (författare)
- San Raffaele Hospital
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- Fassina, Lorenzo (författare)
- University of Pavia
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- James, Peter (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,University of Turku
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- Coffey, Eleanor (författare)
- University of Turku
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- Rea, Giuseppina (författare)
- CNR Istituto per le Tecnologie Applicate ai Beni Culturali (CNR-ITABC)
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- Visai, Livia (författare)
- University of Pavia
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- Rizzo, Angela Maria (författare)
- University of Milan
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(creator_code:org_t)
- 2022-10-01
- 2022
- Engelska.
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Ingår i: Cellular and Molecular Life Sciences. - : Springer Science and Business Media LLC. - 1420-682X .- 1420-9071. ; 79:10
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Microgravity-induced bone loss is a major concern for space travelers. Ground-based microgravity simulators are crucial to study the effect of microgravity exposure on biological systems and to address the limitations posed by restricted access to real space. In this work, for the first time, we adopt a multidisciplinary approach to characterize the morphological, biochemical, and molecular changes underlying the response of human bone marrow stromal cells to long-term simulated microgravity exposure during osteogenic differentiation. Our results show that osteogenic differentiation is reduced while energy metabolism is promoted. We found novel proteins were dysregulated under simulated microgravity, including CSC1-like protein, involved in the mechanotransduction of pressure signals, and PTPN11, SLC44A1 and MME which are involved in osteoblast differentiation pathways and which may become the focus of future translational projects. The investigation of cell proteome highlighted how simulated microgravity affects a relatively low number of proteins compared to time and/or osteogenic factors and has allowed us to reconstruct a hypothetical pipeline for cell response to simulated microgravity. Further investigation focused on the application of nanomaterials may help to increase understanding of how to treat or minimize the effects of microgravity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Bioimaging
- Bone extracellular matrix
- Cytoskeleton
- Data-independent acquisition
- Human primary cells
- Osteogenic biomarkers
- Proteomics
- Secondary osteoporosis
- Simulated microgravity
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Montagna, Giulia
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Pani, Giuseppe
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Flinkman, Dani
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Cristofaro, Fran ...
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Pascucci, Barbar ...
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Massimino, Luca
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visa fler...
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Lamparelli, Luig ...
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Fassina, Lorenzo
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James, Peter
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Coffey, Eleanor
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Rea, Giuseppina
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Visai, Livia
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Rizzo, Angela Ma ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Cellular and Mol ...
- Av lärosätet
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Lunds universitet