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Mass spectrometry e...
Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol
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- Murillo, Jimmy Rodriguez (författare)
- Lund University
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- Pla, Indira (författare)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups,Skåne University Hospital
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- Goto-Silva, Livia (författare)
- D'Or Institute for Research and Education
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- Nogueira, Fábio C.S. (författare)
- Federal University of Rio de Janeiro
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- Domont, Gilberto B. (författare)
- Federal University of Rio de Janeiro
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- Perez-Riverol, Yasset (författare)
- Wellcome Trust
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- Sánchez, Aniel (författare)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups,Skåne University Hospital
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- Junqueira, Magno (författare)
- Federal University of Rio de Janeiro
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(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- Engelska 4 s.
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697. ; 559, s. 51-54
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) < 20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Biological variation
- Cell culture
- iTRAQ
- Quantitative proteomics
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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