Sökning: onr:"swepub:oai:lup.lub.lu.se:b6a78db8-4f29-4631-a467-0cc39a533ef8" > Reproducible Quanti...
Fältnamn | Indikatorer | Metadata |
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000 | 03801naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:b6a78db8-4f29-4631-a467-0cc39a533ef8 | |
003 | SwePub | |
008 | 160401s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/29949742 URI |
024 | 7 | a https://doi.org/10.1126/scitranslmed.30039892 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Huttenhain, Ruth4 aut |
245 | 1 0 | a Reproducible Quantification of Cancer-Associated Proteins in Body Fluids Using Targeted Proteomics |
264 | 1 | b American Association for the Advancement of Science (AAAS),c 2012 |
520 | a The rigorous testing of hypotheses on suitable sample cohorts is a major limitation in translational research. This is particularly the case for the validation of protein biomarkers; the lack of accurate, reproducible, and sensitive assays for most proteins has precluded the systematic assessment of hundreds of potential marker proteins described in the literature. Here, we describe a high-throughput method for the development and refinement of selected reaction monitoring (SRM) assays for human proteins. The method was applied to generate such assays for more than 1000 cancer-associated proteins, which are functionally related to candidate cancer driver mutations. We used the assays to determine the detectability of the target proteins in two clinically relevant samples: plasma and urine. One hundred eighty-two proteins were detected in depleted plasma, spanning five orders of magnitude in abundance and reaching below a concentration of 10 ng/ml. The narrower concentration range of proteins in urine allowed the detection of 408 proteins. Moreover, we demonstrate that these SRM assays allow reproducible quantification by monitoring 34 biomarker candidates across 83 patient plasma samples. Through public access to the entire assay library, researchers will be able to target their cancer-associated proteins of interest in any sample type using the detectability information in plasma and urine as a guide. The generated expandable reference map of SRM assays for cancer-associated proteins will be a valuable resource for accelerating and planning biomarker verification studies. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
700 | 1 | a Soste, Martin4 aut |
700 | 1 | a Selevsek, Nathalie4 aut |
700 | 1 | a Roest, Hannes4 aut |
700 | 1 | a Sethi, Atul4 aut |
700 | 1 | a Carapito, Christine4 aut |
700 | 1 | a Farrah, Terry4 aut |
700 | 1 | a Deutsch, Eric W.4 aut |
700 | 1 | a Kusebauch, Ulrike4 aut |
700 | 1 | a Moritz, Robert L.4 aut |
700 | 1 | a Niméus, Emmau Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Bröstcancer Proteogenomik,Forskargrupper vid Lunds universitet,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Breast cancer Proteogenomics,Lund University Research Groups4 aut0 (Swepub:lu)onk-ens |
700 | 1 | a Rinner, Oliver4 aut |
700 | 1 | a Aebersold, Ruedi4 aut |
710 | 2 | a Bröstcancer-genetikb Sektion I4 org |
773 | 0 | t Science Translational Medicined : American Association for the Advancement of Science (AAAS)g 4:142, s. 94-142q 4:142<94-142x 1946-6242x 1946-6234 |
856 | 4 | u http://dx.doi.org/10.1126/scitranslmed.3003989y FULLTEXT |
856 | 4 | u https://europepmc.org/articles/pmc3766734?pdf=render |
856 | 4 8 | u https://lup.lub.lu.se/record/2994974 |
856 | 4 8 | u https://doi.org/10.1126/scitranslmed.3003989 |
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