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Residues from trans...
Residues from transmembrane helices 3 and 5 participate in leukotriene B-4 binding to BLT1
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
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2006-04-12
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American Chemical Society (ACS),2006
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:b7b782b1-7572-42ad-817d-48c20b172cb5
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https://lup.lub.lu.se/record/409783URI
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https://doi.org/10.1021/bi060076tDOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1927239URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Leukotrienes are inflammatory mediators that bind to seven transmembrane, G-protein-coupled receptors (GPCRs). Here we examine residues from transmembrane helices 3 and 5 of the leukotriene B-4 (LTB4) receptor BLT1 to elucidate how these residues are involved in ligand binding. We have selected these residues on the basis of (1) amino acid sequence analysis, (2) receptor binding and activation studies with a variety of leukotriene-like ligands and recombinant BLT1 receptors, (3) previously published recombinant BLT1 mutants, and (4) a computed model of the active structure of the BLT1 receptor. We propose that LTB4 binds with the polar carboxylate group of LTB4 near the extracellular surface of BLT1 and with the hydrophobic LTB4 tail pointing into the transmembrane regions of the receptor protein. The carboxylate group and the two hydroxyls of LTB4 interact with Arg178 and Glu185 in transmembrane helix 5. Residues from transmembrane helix 3, Val 105 and Ile 108, also line the pocket deeper inside the receptor. LTB4 is becoming increasingly important as an immunomodulator during a number of pathologies, including atherosclerosis. Detailed information about the LTB4 binding mechanism, and the receptor residues involved, will hopefully aid in the design of new immunomodulatory drugs.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bywater, RP
(författare)
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Bristulf, JesperLund University,Lunds universitet,LU Innovation,Sektionen Forskning, samverkan och innovation,Universitetsförvaltningen,Universitetets ledning och förvaltning,Research, Collaboration and Innovation,Central Administration,University Management and Central Administration(Swepub:lu)mphy-jbf
(författare)
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Owman, ChristerLund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)mphy-cow
(författare)
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Haeggstrom, JZKarolinska Institutet
(författare)
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LU InnovationSektionen Forskning, samverkan och innovation
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemistry: American Chemical Society (ACS)45:18, s. 5733-57440006-29601520-4995
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