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Adsorption and Acti...
Adsorption and Activity of Trichoderma reesei Cellobiohydrolase I, Endoglucanase II, and the Corresponding Core Proteins on Steam Pretreated Willow
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Kotiranta, Pia (författare)
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Karlsson, Johan (författare)
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Siika-Aho, Matti (författare)
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Medve, József (författare)
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Viikari, Liisa (författare)
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- Tjerneld, Folke (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Tenkanen, Maija (författare)
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(creator_code:org_t)
- 1999
- 1999
- Engelska.
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Ingår i: Applied Biochemistry and Biotechnology. - 1559-0291. ; 81:2, s. 81-90
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Abstract
Ämnesord
Stäng
- The adsorption and the hydrolytic action of purified cellulases of Trichoderma reesei, namely, cellobiohydrolase I (CBH I), endoglucanase II (EG II), and their core proteins, on steam-pretreated willow were compared. The two enzymes differed clearly in their adsorption and hydrolytic behavior. CBH I required the cellulose-binding domain (CBD) for efficient adsorption and hydrolysis, whereas EG II was able to adsorb to steam pretreated willow without its CBD. Absence of the CBD decreased the hydrolysis of cellulose by EG II, but the decrease was less pronounced than with CBH I. A linear relationship was observed between the amount of enzyme adsorbed and the degree of hydrolysis of cellulose only for CBH I. EG II and EG II core appeared to be able to hydrolyze only 1 to 2% of the substrate regardless of the amount of protein adsorbed.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Adsorption
- cellulose hydrolysis
- cellobiohydrolase
- endoglucanase
- Trichoderma reesei
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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