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Dispersion of viabl...
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Belfrage, PerLund University,Lunds universitet,Pediatrik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Paediatrics (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
(författare)
Dispersion of viable pig liver cells with collagenase
- Artikel/kapitelEngelska1975
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LIBRIS-ID:oai:lup.lub.lu.se:c28dcb10-d200-4cf5-aad0-ba80ce33ab61
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https://lup.lub.lu.se/record/1102592URI
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https://doi.org/10.1016/0024-3205(75)90130-7DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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Börjesson, Bengt
(författare)
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Hägerstrand, Inga
(författare)
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Nilsson, ÅkeLund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-ani
(författare)
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Olsson, Arne
(författare)
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Wiebe, ThomasLund University,Lunds universitet,Pediatrik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Paediatrics (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)pedi-twi
(författare)
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Akesson, BjörnLund University,Lunds universitet,Kirurgi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Surgery (Lund),Section V,Department of Clinical Sciences, Lund,Faculty of Medicine,Skåne University Hospital(Swepub:lu)kc-bak
(författare)
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Pediatrik, LundSektion V
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Life Sciences: Elsevier BV17:8, s. 1219-12251879-06310024-3205
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