Sökning: onr:"swepub:oai:lup.lub.lu.se:c3f3c44c-5e55-4655-a579-7edee4d4ca9e" > A certified plasmid...
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000 | 06426naa a2201273 4500 | |
001 | oai:lup.lub.lu.se:c3f3c44c-5e55-4655-a579-7edee4d4ca9e | |
003 | SwePub | |
008 | 160401s2015 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-247406 | |
009 | oai:prod.swepub.kib.ki.se:130688302 | |
024 | 7 | a https://lup.lub.lu.se/record/45819872 URI |
024 | 7 | a https://doi.org/10.1038/leu.2014.2172 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2474062 URI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1306883022 URI |
040 | a (SwePub)lud (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a White, H4 aut |
245 | 1 0 | a A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR. |
264 | c 2014-07-18 | |
264 | 1 | b Springer Science and Business Media LLC,c 2015 |
520 | a Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μL. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise the numbers of measured transcripts of e14a2 BCR-ABL1 and three control genes; ABL1, BCR and GUSB. The set of 6 plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (http://irmm.jrc.ec.europa.eu; CRM code ERM-AD623a-f).Leukemia accepted article preview online, 18 July 2014; doi:10.1038/leu.2014.217. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng |
700 | 1 | a Deprez, L4 aut |
700 | 1 | a Corbisier, P4 aut |
700 | 1 | a Hall, V4 aut |
700 | 1 | a Lin, F4 aut |
700 | 1 | a Mazoua, S4 aut |
700 | 1 | a Trapmann, S4 aut |
700 | 1 | a Aggerholm, A4 aut |
700 | 1 | a Andrikovics, H4 aut |
700 | 1 | a Akiki, S4 aut |
700 | 1 | a Barbany, Gu Karolinska Institutet4 aut |
700 | 1 | a Boeckx, N4 aut |
700 | 1 | a Bench, A4 aut |
700 | 1 | a Catherwood, M4 aut |
700 | 1 | a Jansson, Mattiasu Uppsala universitet,Medicinsk genetik och genomik4 aut0 (Swepub:uu)mattjans |
700 | 1 | a Chudleigh, S4 aut |
700 | 1 | a Clench, T4 aut |
700 | 1 | a Colomer, D4 aut |
700 | 1 | a Daraio, F4 aut |
700 | 1 | a Dulucq, S4 aut |
700 | 1 | a Farrugia, J4 aut |
700 | 1 | a Fletcher, L4 aut |
700 | 1 | a Foroni, L4 aut |
700 | 1 | a Ganderton, R4 aut |
700 | 1 | a Gerrard, G4 aut |
700 | 1 | a Gineikienė, E4 aut |
700 | 1 | a Hayette, S4 aut |
700 | 1 | a El Housni, H4 aut |
700 | 1 | a Izzo, B4 aut |
700 | 1 | a Jansson, M4 aut |
700 | 1 | a Johnels, Petrau Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)kgen-pha |
700 | 1 | a Jurcek, T4 aut |
700 | 1 | a Kairisto, V4 aut |
700 | 1 | a Kizilors, A4 aut |
700 | 1 | a Kim, D-W4 aut |
700 | 1 | a Lange, T4 aut |
700 | 1 | a Lion, T4 aut |
700 | 1 | a Polakova, K M4 aut |
700 | 1 | a Martinelli, G4 aut |
700 | 1 | a McCarron, S4 aut |
700 | 1 | a Merle, P A4 aut |
700 | 1 | a Milner, B4 aut |
700 | 1 | a Mitterbauer-Hohendanner, G4 aut |
700 | 1 | a Nagar, M4 aut |
700 | 1 | a Nickless, G4 aut |
700 | 1 | a Nomdedéu, J4 aut |
700 | 1 | a Nymoen, D A4 aut |
700 | 1 | a Leibundgut, E O4 aut |
700 | 1 | a Ozbek, U4 aut |
700 | 1 | a Pajič, T4 aut |
700 | 1 | a Pfeifer, H4 aut |
700 | 1 | a Preudhomme, C4 aut |
700 | 1 | a Raudsepp, K4 aut |
700 | 1 | a Romeo, G4 aut |
700 | 1 | a Sacha, T4 aut |
700 | 1 | a Talmaci, R4 aut |
700 | 1 | a Touloumenidou, T4 aut |
700 | 1 | a Van der Velden, V H J4 aut |
700 | 1 | a Waits, P4 aut |
700 | 1 | a Wang, L4 aut |
700 | 1 | a Wilkinson, E4 aut |
700 | 1 | a Wilson, G4 aut |
700 | 1 | a Wren, D4 aut |
700 | 1 | a Zadro, R4 aut |
700 | 1 | a Ziermann, J4 aut |
700 | 1 | a Zoi, K4 aut |
700 | 1 | a Müller, M C4 aut |
700 | 1 | a Hochhaus, A4 aut |
700 | 1 | a Schimmel, H4 aut |
700 | 1 | a Cross, N C P4 aut |
700 | 1 | a Emons, H4 aut |
710 | 2 | a Karolinska Institutetb Medicinsk genetik och genomik4 org |
773 | 0 | t Leukemiad : Springer Science and Business Media LLCg 29:2, s. 369-376q 29:2<369-376x 1476-5551x 0887-6924 |
856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/25036192?dopt=Abstracty FULLTEXT |
856 | 4 | u http://dx.doi.org/10.1038/leu.2014.217y FULLTEXT |
856 | 4 | u https://www.nature.com/articles/leu2014217.pdf |
856 | 4 | u https://doi.org/10.1038/leu.2014.217y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:796781/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://lup.lub.lu.se/record/4581987 |
856 | 4 8 | u https://doi.org/10.1038/leu.2014.217 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-247406 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:130688302 |
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