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Sökning: onr:"swepub:oai:lup.lub.lu.se:c6b2cb42-0c38-479c-8f2e-079460e66d4d" > Ancient bacteria sh...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003553naa a2200505 4500
001oai:lup.lub.lu.se:c6b2cb42-0c38-479c-8f2e-079460e66d4d
003SwePub
008160401s2007 | |||||||||||000 ||eng|
024a https://lup.lub.lu.se/record/6569652 URI
024a https://doi.org/10.1073/pnas.07067871042 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
100a Johnson, Sarah Stewart4 aut
2451 0a Ancient bacteria show evidence of DNA repair
264 c 2007-09-04
264 1b Proceedings of the National Academy of Sciences,c 2007
520 a Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability.
650 7a NATURVETENSKAPx Geovetenskap och miljövetenskapx Naturgeografi0 (SwePub)105072 hsv//swe
650 7a NATURAL SCIENCESx Earth and Related Environmental Sciencesx Physical Geography0 (SwePub)105072 hsv//eng
653 a DNA damage
653 a long-term microbial survival
653 a metabolic activity
700a Hebsgaard, Martin B.4 aut
700a Christensen, Torbenu Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science4 aut0 (Swepub:lu)ekol-tch
700a Mastepanov, Mikhailu Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science4 aut0 (Swepub:lu)ple-mma
700a Nielsen, Rasmus4 aut
700a Munch, Kasper4 aut
700a Brand, Tina4 aut
700a Thomas, M.4 aut
700a Gilbert, P.4 aut
700a Zuber, Maria T.4 aut
700a Bunce, Michael4 aut
700a Ronn, Regin4 aut
700a Gilichinsky, David4 aut
700a Froese, Duane4 aut
700a Willerslev, Eske4 aut
710a Institutionen för naturgeografi och ekosystemvetenskapb Naturvetenskapliga fakulteten4 org
773t Proceedings of the National Academy of Sciencesd : Proceedings of the National Academy of Sciencesg 104:36, s. 14401-14405q 104:36<14401-14405x 1091-6490x 0027-8424
856u http://dx.doi.org/10.1073/pnas.0706787104x freey FULLTEXT
856u https://europepmc.org/articles/pmc1958816?pdf=render
8564 8u https://lup.lub.lu.se/record/656965
8564 8u https://doi.org/10.1073/pnas.0706787104

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