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Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands.
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Yun, Junxian (författare)
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Cheng, Xiuhong (författare)
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Ye, Jialei (författare)
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Shen, Shaochuan (författare)
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Yang, Gensheng (författare)
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Yao, Kejian (författare)
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- Kirsebom, Harald (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Lin, Dong-Qiang (författare)
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Guan, Yi-Xin (författare)
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Yao, Shan-Jing (författare)
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Journal of Chromatography A. - : Elsevier BV. - 0021-9673. ; 1381, s. 173-183
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The preparation and characterization of mixed-mode adsorbents for a typical separation purpose are of great importance in bioseparation areas. In this work, we prepared a new monolithic cryogel with a combination of ion-exchange and hydrophobic functions by employing benzyl-quaternary amine groups. The fundamental cryogel properties, protein equilibrium adsorption isotherm and chromatographic adsorption in the cryogel were measured experimentally. The results showed that, by using bovine serum album as the model protein, the dual functional cryogel has protein binding capability even in salt solution and the buffer with pH close or below the protein isoelectric point due to both the electrostatic and hydrophobic interactions. A capillary-based adsorption model was developed, which provided satisfied insights of the microstructure, axial dispersion, mass transfer as well as protein adsorption characteristics within the cryogel bed. The chromatographic isolation of bioactive proteins from rabbit blood serum was carried out by the cryogel. Immunoglobulin G antibody with a purity of 98.2% and albumin with a purity of 96.8% were obtained, indicating that the cryogel could be an interesting and promising adsorbent in bioseparation areas.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
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Yun, Junxian
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Cheng, Xiuhong
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Ye, Jialei
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Shen, Shaochuan
-
Yang, Gensheng
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Yao, Kejian
-
visa fler...
-
Kirsebom, Harald
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Lin, Dong-Qiang
-
Guan, Yi-Xin
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Yao, Shan-Jing
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
- Artiklar i publikationen
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Journal of Chrom ...
- Av lärosätet
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Lunds universitet