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The microheterogeneity of desialylated α1-antichymotrypsin : the occurrence of two amino-terminal isoforms, one lacking a His-Pro dipeptide
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- Lindmark, Bertil (författare)
- Skåne University Hospital
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- Lilja, Hans (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Skåne University Hospital
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- Alm, Ragnar (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Skåne University Hospital
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- Eriksson, Sten (författare)
- Skåne University Hospital
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(creator_code:org_t)
- Elsevier BV, 1989
- Engelska.
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Ingår i: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. - : Elsevier BV. - 0167-4838. ; 997:1-2, s. 90-95
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- ACT (α1-antichymotrypsin), a serine antiproteinase with specificity against neutrophil cathepsin G, is homologous with α1-antitrypsin, plasminogen activator inhibitor and angiotensinogen, all with known amino-terminal microheterogeneity. Here we report that the two predominant isoforms of desialylated ACT obtained on isoelectric focusing correspond to a microheterogeneity at the amino terminus of ACT: one isoform (His-Pro-Asn-Ser-Pro-) and a two residues shorter isoform (Asn-Ser-Pro-). The relative occurrence of the two isoforms was comparable both in normal plasma, acute-phase plasma and plasma from subjects with heterozygous familial ACT deficiency. When desialyllated ACT, isolated by affinity chromatography from ACT-deficient, normal or acute-phase plasma, was compared with regard to mass and charge microheterogeneity, we found no significant differences in either respect. Nor was the isoform pattern of desialylated plasma from patients with rheumatoid arthritis different. Although the occurrence of heterozygous familial ACT deficiency implies genotypic variation, isolated ACT from patients with the trait was not found to exhibit any phenotypic variation detectable by standard electrophoretic methods.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Nyckelord
- Amino terminal amino acid sequence
- Isoelectric focusing
- Microheterogeneity
- Serpin
- α-Antichymotrypsin
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)