Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models

Topping, Louise M.Kennedy Institute of Rheumatology,University of Oxford (författare)

Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for in vivo research involving animal models of autoimmune disease and in vaccine development.

Romero-Castillo, LauraKarolinska Institutet,Karolinska Institute (författare)
Urbonaviciute, VilmaKarolinska Institutet,Karolinska Institute (författare)
Bolinsson, HansLund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)food-hab (författare)
Clanchy, Felix I.University of Oxford,Kennedy Institute of Rheumatology (författare)
Holmdahl, RikardKarolinska Institutet,Karolinska Institute(Swepub:lu)infl-rho (författare)
Bäckström, B. ThomasLund University,Lunds universitet,Neurokirurgi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Neurosurgery,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,BTB Emulsions AB(Swepub:lu)th7133ba (författare)
Williams, Richard O.Kennedy Institute of Rheumatology,University of Oxford (författare)
Kennedy Institute of RheumatologyUniversity of Oxford (creator_code:org_t)
Karolinska Institutet
Karolinska Institutet

Av författaren/redakt...: Topping, Louise ...; Romero-Castillo, ...; Urbonaviciute, V ...; Bolinsson, Hans; Clanchy, Felix I ...; Holmdahl, Rikard; visa fler...; Bäckström, B. Th ...; Williams, Richar ...; visa färre...

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