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Leaky Optoelectrica...
Leaky Optoelectrical Fiber for Optogenetic Stimulation and Electrochemical Detection of Dopamine Exocytosis from Human Dopaminergic Neurons
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- Vasudevan, Shashank (författare)
- Technical University of Denmark
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- Kajtez, Janko (författare)
- Technical University of Denmark
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- Bunea, Ada Ioana (författare)
- Technical University of Denmark
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- Gonzalez-Ramos, Ana (författare)
- Lund University,Lunds universitet,Experimentell Epilepsi,Forskargrupper vid Lunds universitet,Experimental Epilepsy Group,Lund University Research Groups,Skåne University Hospital
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- Ramos-Moreno, Tania (författare)
- Lund University,Lunds universitet,Epilepsicentrum,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Epilepsy Center,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Heiskanen, Arto (författare)
- Technical University of Denmark
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- Kokaia, Merab (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Epilepsicentrum,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Other operations, LTH,Faculty of Engineering, LTH,Epilepsy Center,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Skåne University Hospital
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- Larsen, Niels B. (författare)
- Technical University of Denmark
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- Martínez-Serrano, Alberto (författare)
- Autonomous University of Madrid
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- Keller, Stephan S. (författare)
- Technical University of Denmark
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- Emnéus, Jenny (författare)
- Technical University of Denmark
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(creator_code:org_t)
- 2019-10-16
- 2019
- Engelska.
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Ingår i: Advanced Science. - : Wiley. - 2198-3844. ; 6:24
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://onlinelibrar...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In Parkinson's disease, the degeneration of dopaminergic neurons in substantia nigra leads to a decrease in the physiological levels of dopamine in striatum. The existing dopaminergic therapies effectively alleviate the symptoms, albeit they do not revert the disease progression and result in significant adverse effects. Transplanting dopaminergic neurons derived from stem cells could restore dopamine levels without additional motor complications. However, the transplanted cells disperse in vivo and it is not possible to stimulate them on demand to modulate dopamine release to prevent dyskinesia. In order to address these issues, this paper presents a multifunctional leaky optoelectrical fiber for potential neuromodulation and as a cell substrate for application in combined optogenetic stem cell therapy. Pyrolytic carbon coated optical fibers are laser ablated to pattern micro-optical windows to permit light leakage over a large area. The pyrolytic carbon acts as an excellent electrode for the electrochemical detection of dopamine. Human neural stem cells are genetically modified to express the light sensitive opsin channelrhodopsin-2 and are differentiated into dopaminergic neurons on the leaky optoelectrical fiber. Finally, light leaking from the micro-optical windows is used to stimulate the dopaminergic neurons resulting in the release of dopamine that is detected in real-time using chronoamperometry.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- dopamine exocytosis
- human neural stem cells
- leaky optical fibers
- optogenetics
- pyrolysis
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Vasudevan, Shash ...
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Kajtez, Janko
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Bunea, Ada Ioana
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Gonzalez-Ramos, ...
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Ramos-Moreno, Ta ...
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Heiskanen, Arto
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visa fler...
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Kokaia, Merab
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Larsen, Niels B.
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Martínez-Serrano ...
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Keller, Stephan ...
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Emnéus, Jenny
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Advanced Science
- Av lärosätet
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Lunds universitet