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Injection of high d...
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Pingel, JessicaUniversity of Copenhagen
(författare)
Injection of high dose botulinum-toxin A leads to impaired skeletal muscle function and damage of the fibrilar and non-fibrilar structures
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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2017-11-07
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Springer Science and Business Media LLC,2017
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:d42176e1-cc94-44b9-8c45-a32562b75aa8
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https://lup.lub.lu.se/record/d42176e1-cc94-44b9-8c45-a32562b75aa8URI
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https://doi.org/10.1038/s41598-017-14997-3DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
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Botulinum-toxin A (BoNT/A) is used for a wide range of conditions. Intramuscular administration of BoNT/A inhibits the release of acetylcholine at the neuromuscular junction from presynaptic motor neurons causing muscle-paralysis. The aim of the present study was to investigate the effect of high dose intramuscular BoNT/A injections (6 UI = 60 pg) on muscle tissue. The gait pattern of the rats was significantly affected 3 weeks after BoNT/A injection. The ankle joint rotated externally, the rats became flat footed, and the stride length decreased after BoNT/A injection. Additionally, there was clear evidence of microstructural changes on the tissue level by as evidenced by 3D imaging of the muscles by Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM). Both the fibrillar and the non-fibrillar tissues were affected. The volume fraction of fibrillary tissue was reduced significantly and the non-fibrillar tissue increased. This was accompanied by a loss of the linear structure of the muscle tissue. Furthermore, gene expression analysis showed a significant upregulation of COL1A1, MMP-2, TGF-b1, IL-6, MHCIIA and MHCIIx in the BoNT/A injected leg, while MHVIIB was significantly downregulated. In conclusion: The present study reveals that high dose intramuscular BoNT/A injections cause microstructural damage of the muscle tissue, which contributes to impaired gait.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Nielsen, Mikkel SchouUniversity of Copenhagen
(författare)
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Lauridsen, TorstenUniversity of Copenhagen
(författare)
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Rix, KristianUniversity of Copenhagen(Swepub:lu)kr1387ri
(författare)
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Bech, MartinLund University,Lunds universitet,Medicinsk strålningsfysik, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medical Radiation Physics, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)med-mbh
(författare)
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Alkjaer, TineUniversity of Copenhagen
(författare)
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Andersen, Ida TorpUniversity of Copenhagen
(författare)
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Nielsen, Jens BoUniversity of Copenhagen
(författare)
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Feidenhansl, R.University of Copenhagen
(författare)
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University of CopenhagenMedicinsk strålningsfysik, Lund
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scientific Reports: Springer Science and Business Media LLC7:12045-2322
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