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The Mini-Organo : A...
The Mini-Organo : A rapid high-throughput 3D coculture organotypic assay for oncology screening and drug development
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- Chitty, Jessica L. (författare)
- The Kinghorne Cancer Centre
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- Skhinas, Joanna N. (författare)
- The Kinghorne Cancer Centre
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- Filipe, Elysse C. (författare)
- University of New South Wales
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- Wang, Shan (författare)
- Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancer and matrix remodelling,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Cupello, Carmen Rodriguez (författare)
- Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancer and matrix remodelling,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Grant, Rhiannon D. (författare)
- Garvan Institute of Medical Research
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- Yam, Michelle (författare)
- Garvan Institute of Medical Research
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- Papanicolaou, Michael (författare)
- Garvan Institute of Medical Research
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- Major, Gretel (författare)
- Garvan Institute of Medical Research
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- Zaratzian, Anaiis (författare)
- Garvan Institute of Medical Research
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- Da Silva, Andrew M. (författare)
- Garvan Institute of Medical Research
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- Tayao, Michael (författare)
- Garvan Institute of Medical Research
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- Vennin, Claire (författare)
- Netherlands Cancer Institute
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- Timpson, Paul (författare)
- University of New South Wales
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- Madsen, Chris D. (författare)
- Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancer and matrix remodelling,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Cox, Thomas R. (författare)
- Garvan Institute of Medical Research
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(creator_code:org_t)
- 2019-08
- 2020
- Engelska.
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Ingår i: Cancer Reports. - : Wiley. - 2573-8348. ; 3:1
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://onlinelibrar...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Background: The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease. Aim: The development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to several weeks), and work-intensive with often limited scalability. Our aim was to develop a fast, high-throughput, scalable 3D organotypic assay for use in oncology screening and drug development. Methods and results Here, we describe a modified 96-well organotypic assay, the "Mini-Organo," which can be easily completed within 5 days. We demonstrate its application in a wide range of mouse and human cancer biology approaches including evaluation of stromal cell 3D ECM remodelling, 3D cancer cell invasion, and the assessment of efficacy of potential anticancer therapeutic targets. Furthermore, the organotypic assay described is highly amenable to customisation using different cell types under diverse experimental conditions. Conclusions: The Mini-Organo high-throughput 3D organotypic assay allows the rapid screening of potential cancer therapeutics in human and mouse models in a time-efficient manner.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- 3D model
- cancer-associated fibroblast
- coculture
- drug screening
- extracellular matrix
- organotypic
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Chitty, Jessica ...
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Skhinas, Joanna ...
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Filipe, Elysse C ...
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Wang, Shan
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Cupello, Carmen ...
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Grant, Rhiannon ...
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visa fler...
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Yam, Michelle
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Papanicolaou, Mi ...
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Major, Gretel
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Zaratzian, Anaii ...
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Da Silva, Andrew ...
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Tayao, Michael
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Vennin, Claire
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Timpson, Paul
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Madsen, Chris D.
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Cox, Thomas R.
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Cancer Reports
- Av lärosätet
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Lunds universitet