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The triolein/aqueou...
The triolein/aqueous interface and lipase activity studied by spectroscopic ellipsometry and coarse grained simulations
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- Stamm, Arne (författare)
- Physical Chemistry, Department of Chemistry, Lund University,Novozymes A/S
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- Svendsen, Allan (författare)
- Novozymes A/S
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- Skjold-Jørgensen, Jakob (författare)
- Novozymes A/S
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- Vissing, Thomas (författare)
- Novozymes A/S
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- Berts, Ida (författare)
- Jülich Research Centre
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- Nylander, Tommy (författare)
- Lund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- Engelska 7 s.
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Ingår i: Chemistry and Physics of Lipids. - : Elsevier BV. - 0009-3084 .- 1873-2941. ; 211, s. 37-43
- Relaterad länk:
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http://dx.doi.org/10...
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http://linkinghub.el...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- In spite of the importance of the triglyceride aqueous interface for processes like emulsification, surfactant interactions and lipase activity, relatively little is known about this interface compared to that between alkanes and water. Here, the contact between triolein and water was investigated in terms of water inclusion in the oil phase and orientation of the molecules at the interface. Coarse grained models of triglycerides in contact with water were constructed and correlated with experimental results of the changes in thickness and refractive index, obtained using spectroscopic ellipsometry of spin-coated triolein films. The topography of the layer was revealed by atomic force microscopy. Dry triolein and a triolein sample after equilibration with water were also compared structurally using small-angle X-ray scattering. Additionally, the kinetics of adsorption/activity of three different variants of the Thermomyces lanuginosus lipase (TLL) were investigated. The results show that uptake of water in the triolein phase leads to increase in thickness of the layer. The observed increase of thickness was further enhanced by an active lipase but reduced when an inactive mutant of the enzyme was applied.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Coarse graining simulations
- Lipase activity
- Oil phase hydration
- Small-angle x-ray scattering
- Spectroscopic ellipsometry
- Thermomyces lanuginosus lipase
- Triolein
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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