Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: onr:"swepub:oai:openarchive.ki.se:10616/48411" > Amelioration of sys...

1 av 1
Föregående post
Nästa post
Till träfflistan

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		04043naa a2200673 4500
001		oai:openarchive.ki.se:10616/48411
003		SwePub
008		240410s2021 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:147830459
022		a 2157-846X
024	7	a 10616/484112 hdl
024	7	a http://hdl.handle.net/10616/484112 URI
024	7	a https://doi.org/10.1038/s41551-021-00792-z2 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1478304592 URI
040		a (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Gupta, Dhanuu Karolinska Institutet4 aut
245	1 0	a Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles
264		c 2021-10-06
264	1	a Stockholm :b Karolinska Institutet, Dept of Laboratory Medicine,c 2021
338		a electronic2 rdacarrier
520		a Extracellular vesicles (EVs) can be functionalized to display specific protein receptors on their surface. However, surface-display technology typically labels only a small fraction of the EV population. Here, we show that the joint display of two different therapeutically relevant protein receptors on EVs can be optimized by systematically screening EV-loading protein moieties. We used cytokine-binding domains derived from tumour necrosis factor receptor 1 (TNFR1) and interleukin-6 signal transducer (IL-6ST), which can act as decoy receptors for the pro-inflammatory cytokines tumour necrosis factor alpha (TNF-α) and IL-6, respectively. We found that the genetic engineering of EV-producing cells to express oligomerized exosomal sorting domains and the N-terminal fragment of syntenin (a cytosolic adaptor of the single transmembrane domain protein syndecan) increased the display efficiency and inhibitory activity of TNFR1 and IL-6ST and facilitated their joint display on EVs. In mouse models of systemic inflammation, neuroinflammation and intestinal inflammation, EVs displaying the cytokine decoys ameliorated the disease phenotypes with higher efficacy as compared with clinically approved biopharmaceutical agents targeting the TNF-α and IL-6 pathways.
700	1	a Wiklander, Oscar P Bu Karolinska Institutet4 aut
700	1	a Görgens, Andréu Karolinska Institutet4 aut
700	1	a Conceição, Mariana4 aut
700	1	a Corso, Giuliau Karolinska Institutet4 aut
700	1	a Liang, Xiumingu Karolinska Institutet4 aut
700	1	a Seow, Yiqi4 aut
700	1	a Balusu, Sriram4 aut
700	1	a Feldin, Ulrika4 aut
700	1	a Bostancioglu, Beklem4 aut
700	1	a Jawad, Rimu Karolinska Institutet4 aut
700	1	a Mamand, Doste Ru Karolinska Institutet4 aut
700	1	a Lee, Yi Xin Fiona4 aut
700	1	a Hean, Justin4 aut
700	1	a Mäger, Imre4 aut
700	1	a Roberts, Thomas C4 aut
700	1	a Gustafsson, Manuelau Karolinska Institutet4 aut
700	1	a Mohammad, Dara Ku Karolinska Institutet4 aut
700	1	a Sork, Helenau Karolinska Institutet4 aut
700	1	a Backlund, Alexandra4 aut
700	1	a Lundin, Per4 aut
700	1	a de Fougerolles, Antonin4 aut
700	1	a Smith, C I Edvardu Karolinska Institutet4 aut
700	1	a Wood, Matthew J A4 aut
700	1	a Vandenbroucke, Roosmarijn E4 aut
700	1	a Nordin, Joel Zu Karolinska Institutet4 aut
700	1	a El-Andaloussi, Samiru Karolinska Institutet4 aut
710	2	a Karolinska Institutet
710	2	a Karolinska Institutet
710	2	a Karolinska Institutet4 org
773	0	t Nature Biomedical Engineeringd Stockholm : Karolinska Institutet, Dept of Laboratory Medicinex 2157-846X
856	4	u http://hdl.handle.net/10616/48411x primaryx Object in contextx freey FULLTEXT
856	4 8	u http://hdl.handle.net/10616/48411
856	4 8	u https://doi.org/10.1038/s41551-021-00792-z
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:147830459

Hitta via bibliotek

Nature Biomedical Engineering (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

1 av 1
Föregående post
Nästa post
Till träfflistan

Hitta mer i SwePub

Artiklar i publikationen: Nature Biomedica ...

Av lärosätet: Karolinska Institutet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se