Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:128248915" >
Large-scale hypomet...
Large-scale hypomethylated blocks associated with Epstein-Barr virus-induced B-cell immortalization
-
Hansen, KD (författare)
-
Sabunciyan, S (författare)
-
Langmead, B (författare)
-
visa fler...
-
- Nagy, N (författare)
- Karolinska Institutet
-
Curley, R (författare)
-
- Klein, G (författare)
- Karolinska Institutet
-
- Klein, E (författare)
- Karolinska Institutet
-
- Salamon, D (författare)
- Karolinska Institutet
-
Feinberg, AP (författare)
-
visa färre...
-
(creator_code:org_t)
- 2013-09-25
- 2014
- Engelska.
-
Ingår i: Genome research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 24:2, s. 177-184
- Relaterad länk:
-
http://genome.cshlp....
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Altered DNA methylation occurs ubiquitously in human cancer from the earliest measurable stages. A cogent approach to understanding the mechanism and timing of altered DNA methylation is to analyze it in the context of carcinogenesis by a defined agent. Epstein-Barr virus (EBV) is a human oncogenic herpesvirus associated with lymphoma and nasopharyngeal carcinoma, but also used commonly in the laboratory to immortalize human B-cells in culture. Here we have performed whole-genome bisulfite sequencing of normal B-cells, activated B-cells, and EBV-immortalized B-cells from the same three individuals, in order to identify the impact of transformation on the methylome. Surprisingly, large-scale hypomethylated blocks comprising two-thirds of the genome were induced by EBV immortalization but not by B-cell activation per se. These regions largely corresponded to hypomethylated blocks that we have observed in human cancer, and they were associated with gene-expression hypervariability, similar to human cancer, and consistent with a model of epigenomic change promoting tumor cell heterogeneity. We also describe small-scale changes in DNA methylation near CpG islands. These results suggest that methylation disruption is an early and critical step in malignant transformation.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas