Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:133307121" >
G3BP-Caprin1-USP10 ...
G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits
-
Kedersha, N (författare)
-
Panas, MD (författare)
-
Achorn, CA (författare)
-
visa fler...
-
Lyons, S (författare)
-
Tisdale, S (författare)
-
Hickman, T (författare)
-
Thomas, M (författare)
-
Lieberman, J (författare)
-
- McInerney, GM (författare)
- Karolinska Institutet
-
Ivanov, P (författare)
-
Anderson, P (författare)
-
visa färre...
-
(creator_code:org_t)
- Rockefeller University Press, 2016
- 2016
- Engelska.
-
Ingår i: The Journal of cell biology. - : Rockefeller University Press. - 1540-8140 .- 0021-9525. ; 212:7, s. 845-860
- Relaterad länk:
-
https://rupress.org/...
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Mammalian stress granules (SGs) contain stalled translation preinitiation complexes that are assembled into discrete granules by specific RNA-binding proteins such as G3BP. We now show that cells lacking both G3BP1 and G3BP2 cannot form SGs in response to eukaryotic initiation factor 2α phosphorylation or eIF4A inhibition, but are still SG-competent when challenged with severe heat or osmotic stress. Rescue experiments using G3BP1 mutants show that G3BP1-F33W, a mutant unable to bind G3BP partner proteins Caprin1 or USP10, rescues SG formation. Caprin1/USP10 binding to G3BP is mutually exclusive: Caprin binding promotes, but USP10 binding inhibits, SG formation. G3BP interacts with 40S ribosomal subunits through its RGG motif, which is also required for G3BP-mediated SG formation. We propose that G3BP mediates the condensation of SGs by shifting between two different states that are controlled by binding to Caprin1 or USP10.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Kedersha, N
-
Panas, MD
-
Achorn, CA
-
Lyons, S
-
Tisdale, S
-
Hickman, T
-
visa fler...
-
Thomas, M
-
Lieberman, J
-
McInerney, GM
-
Ivanov, P
-
Anderson, P
-
visa färre...
- Artiklar i publikationen
-
The Journal of c ...
- Av lärosätet
-
Karolinska Institutet