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Metabolomics analys...
Metabolomics analysis identifies different metabotypes of asthma severity
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Reinke, SN (författare)
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Gallart-Ayala, H (författare)
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- Gomez, C (författare)
- Karolinska Institutet
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- Checa, A (författare)
- Karolinska Institutet
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Fauland, A (författare)
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- Naz, S (författare)
- Karolinska Institutet
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Kamleh, MA (författare)
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Djukanovic, R (författare)
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Hinks, TSC (författare)
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- Wheelock, CE (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2017-03-29
- 2017
- Engelska.
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Ingår i: The European respiratory journal. - : European Respiratory Society (ERS). - 1399-3003 .- 0903-1936. ; 49:3
- Relaterad länk:
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https://erj.ersjourn...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- In this study, we sought to determine whether asthma has a metabolic profile and whether this profile is related to disease severity.We characterised the serum from 22 healthy individuals and 54 asthmatics (12 mild, 20 moderate, 22 severe) using liquid chromatography–high-resolution mass spectrometry-based metabolomics. Selected metabolites were confirmed by targeted mass spectrometry assays of eicosanoids, sphingolipids and free fatty acids.We conclusively identified 66 metabolites; 15 were significantly altered with asthma (p≤0.05). Levels of dehydroepiandrosterone sulfate, cortisone, cortisol, prolylhydroxyproline, pipecolate and N-palmitoyltaurine correlated significantly (p<0.05) with inhaled corticosteroid dose, and were further shifted in individuals treated with oral corticosteroids. Oleoylethanolamide increased with asthma severity independently of steroid treatment (p<0.001). Multivariate analysis revealed two patterns: 1) a mean difference between controls and patients with mild asthma (p=0.025), and 2) a mean difference between patients with severe asthma and all other groups (p=1.7×10−4). Metabolic shifts in mild asthma, relative to controls, were associated with exogenous metabolites (e.g. dietary lipids), while those in moderate and severe asthma (e.g. oleoylethanolamide, sphingosine-1-phosphate, N-palmitoyltaurine) were postulated to be involved in activating the transient receptor potential vanilloid type 1 (TRPV1) receptor, driving TRPV1-dependent pathogenesis in asthma.Our findings suggest that asthma is characterised by a modest systemic metabolic shift in a disease severity-dependent manner, and that steroid treatment significantly affects metabolism.
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Reinke, SN
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Gallart-Ayala, H
-
Gomez, C
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Checa, A
-
Fauland, A
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Naz, S
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visa fler...
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Kamleh, MA
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Djukanovic, R
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Hinks, TSC
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Wheelock, CE
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visa färre...
- Artiklar i publikationen
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The European res ...
- Av lärosätet
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Karolinska Institutet