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Luciferase Expressi...
Luciferase Expression Allows Bioluminescence Imaging But Imposes Limitations on the Orthotopic Mouse (4T1) Model of Breast Cancer
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Baklaushev, VP (författare)
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Kilpelainen, A (författare)
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- Petkov, S (författare)
- Karolinska Institutet
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Abakumov, MA (författare)
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Grinenko, NF (författare)
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Yusubalieva, GM (författare)
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Latanova, AA (författare)
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Gubskiy, IL (författare)
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Zabozlaev, FG (författare)
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Starodubova, ES (författare)
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Abakumova, TO (författare)
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- Isaguliants, MG (författare)
- Karolinska Institutet
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Chekhonin, VP (författare)
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(creator_code:org_t)
- 2017-08-10
- 2017
- Engelska.
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1, s. 7715-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Implantation of reporter-labeled tumor cells in an immunocompetent host involves a risk of their immune elimination. We have studied this effect in a mouse model of breast cancer after the orthotopic implantation of mammary gland adenocarcinoma 4T1 cells genetically labelled with luciferase (Luc). Mice were implanted with 4T1 cells and two derivative Luc-expressing clones 4T1luc2 and 4T1luc2D6 exhibiting equal in vitro growth rates. In vivo, the daughter 4T1luc2 clone exhibited nearly the same, and 4T1luc2D6, a lower growth rate than the parental cells. The metastatic potential of 4T1 variants was assessed by magnetic resonance, bioluminescent imaging, micro-computed tomography, and densitometry which detected 100-μm metastases in multiple organs and bones at the early stage of their development. After 3–4 weeks, 4T1 generated 11.4 ± 2.1, 4T1luc2D6, 4.5 ± 0.6; and 4T1luc2, <1 metastases per mouse, locations restricted to lungs and regional lymph nodes. Mice bearing Luc-expressing tumors developed IFN-γ response to the dominant CTL epitope of Luc. Induced by intradermal DNA-immunization, such response protected mice from the establishment of 4T1luc2-tumors. Our data show that natural or induced cellular response against the reporter restricts growth and metastatic activity of the reporter-labelled tumor cells. Such cells represent a powerful instrument for improving immunization technique for cancer vaccine applications.
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Baklaushev, VP
-
Kilpelainen, A
-
Petkov, S
-
Abakumov, MA
-
Grinenko, NF
-
Yusubalieva, GM
-
visa fler...
-
Latanova, AA
-
Gubskiy, IL
-
Zabozlaev, FG
-
Starodubova, ES
-
Abakumova, TO
-
Isaguliants, MG
-
Chekhonin, VP
-
visa färre...
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Karolinska Institutet