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Age-dependent incre...
Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health
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Heid, J (författare)
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Cencioni, C (författare)
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Ripa, R (författare)
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visa fler...
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Baumgart, M (författare)
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Atlante, S (författare)
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Milano, G (författare)
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Scopece, A (författare)
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Kuenne, C (författare)
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Guenther, S (författare)
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- Azzimato, V (författare)
- Karolinska Institutet
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Farsetti, A (författare)
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Rossi, G (författare)
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Braun, T (författare)
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Pompilio, G (författare)
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Martelli, F (författare)
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Zeiher, AM (författare)
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Cellerino, A (författare)
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Gaetano, C (författare)
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Spallotta, F (författare)
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visa färre...
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(creator_code:org_t)
- 2017-12-04
- 2017
- Engelska.
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1, s. 16839-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- The short-lived turquoise killifish Nothobranchius furzeri (Nfu) is a valid model for aging studies. Here, we investigated its age-associated cardiac function. We observed oxidative stress accumulation and an engagement of microRNAs (miRNAs) in the aging heart. MiRNA-sequencing of 5 week (young), 12–21 week (adult) and 28–40 week (old) Nfu hearts revealed 23 up-regulated and 18 down-regulated miRNAs with age. MiR-29 family turned out as one of the most up-regulated miRNAs during aging. MiR-29 family increase induces a decrease of known targets like collagens and DNA methyl transferases (DNMTs) paralleled by 5´methyl-cytosine (5mC) level decrease. To further investigate miR-29 family role in the fish heart we generated a transgenic zebrafish model where miR-29 was knocked-down. In this model we found significant morphological and functional cardiac alterations and an impairment of oxygen dependent pathways by transcriptome analysis leading to hypoxic marker up-regulation. To get insights the possible hypoxic regulation of miR-29 family, we exposed human cardiac fibroblasts to 1% O2 levels. In hypoxic condition we found miR-29 down-modulation responsible for the accumulation of collagens and 5mC. Overall, our data suggest that miR-29 family up-regulation might represent an endogenous mechanism aimed at ameliorating the age-dependent cardiac damage leading to hypertrophy and fibrosis.
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- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Heid, J
-
Cencioni, C
-
Ripa, R
-
Baumgart, M
-
Atlante, S
-
Milano, G
-
visa fler...
-
Scopece, A
-
Kuenne, C
-
Guenther, S
-
Azzimato, V
-
Farsetti, A
-
Rossi, G
-
Braun, T
-
Pompilio, G
-
Martelli, F
-
Zeiher, AM
-
Cellerino, A
-
Gaetano, C
-
Spallotta, F
-
visa färre...
- Artiklar i publikationen
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Scientific repor ...
- Av lärosätet
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Karolinska Institutet