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Promoter Usage and ...
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Alhendi, AMN
(författare)
Promoter Usage and Dynamics in Vascular Smooth Muscle Cells Exposed to Fibroblast Growth Factor-2 or Interleukin-1β
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
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2018-09-03
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Springer Science and Business Media LLC,2018
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:139087231
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http://kipublications.ki.se/Default.aspx?queryparsed=id:139087231URI
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https://doi.org/10.1038/s41598-018-30702-4DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Smooth muscle cells (SMC) in blood vessels are normally growth quiescent and transcriptionally inactive. Our objective was to understand promoter usage and dynamics in SMC acutely exposed to a prototypic growth factor or pro-inflammatory cytokine. Using cap analysis gene expression (FANTOM5 project) we report differences in promoter dynamics for immediate-early genes (IEG) and other genes when SMC are exposed to fibroblast growth factor-2 or interleukin-1β. Of the 1871 promoters responding to FGF2 or IL-1β considerably more responded to FGF2 (68.4%) than IL-1β (18.5%) and 13.2% responded to both. Expression clustering reveals sets of genes induced, repressed or unchanged. Among IEG responding rapidly to FGF2 or IL-1β were FOS, FOSB and EGR-1, which mediates human SMC migration. Motif activity response analysis (MARA) indicates most transcription factor binding motifs in response to FGF2 were associated with a sharp induction at 1 h, whereas in response to IL-1β, most motifs were associated with a biphasic change peaking generally later. MARA revealed motifs for FOS_FOS{B,L1}_JUN{B,D} and EGR-1..3 in the cluster peaking 1 h after FGF2 exposure whereas these motifs were in clusters peaking 1 h or later in response to IL-1β. Our findings interrogating CAGE data demonstrate important differences in promoter usage and dynamics in SMC exposed to FGF2 or IL-1β.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Patrikakis, M
(författare)
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Daub, COKarolinska Institutet
(författare)
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Kawaji, H
(författare)
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Itoh, M
(författare)
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de Hoon, M
(författare)
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Carninci, P
(författare)
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Hayashizaki, Y
(författare)
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Arner, E
(författare)
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Khachigian, LM
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scientific reports: Springer Science and Business Media LLC8:1, s. 13164-2045-2322
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Alhendi, AMN
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Patrikakis, M
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Daub, CO
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Kawaji, H
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Itoh, M
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de Hoon, M
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visa fler...
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Carninci, P
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Hayashizaki, Y
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Arner, E
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Khachigian, LM
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visa färre...
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Scientific repor ...
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Karolinska Institutet