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PET imaging shows n...
PET imaging shows no changes in TSPO brain density after IFN-α immune challenge in healthy human volunteers
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Nettis, MA (författare)
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Veronese, M (författare)
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Nikkheslat, N (författare)
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Mariani, N (författare)
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Lombardo, G (författare)
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Sforzini, L (författare)
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- Enache, D (författare)
- Karolinska Institutet
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Harrison, NA (författare)
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Turkheimer, FE (författare)
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Mondelli, (författare)
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Pariante, CM (författare)
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(creator_code:org_t)
- 2020-03-09
- 2020
- Engelska.
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Ingår i: Translational psychiatry. - : Springer Science and Business Media LLC. - 2158-3188. ; 10:1, s. 89-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- Depression is associated with peripheral inflammation, but its link with brain microglial activity remains unclear. In seven healthy males, we used repeated translocator protein-Positron Emission Tomography (TSPO-PET) dynamic scans with [11C]PBR28 to image brain microglial activation before and 24 h after the immune challenge interferon (IFN)-α. We also investigated the association between changes in peripheral inflammation, changes in microglial activity, and changes in mood. IFN-α administration decreased [11C]PBR28 PET tissue volume of distribution (Vt) across the brain (−20 ± 4%; t6 = 4.1, p = 0.01), but after correction for radioligand free-plasma fraction there were no longer any changes (+23 ± 31%; t = 0.1, p = 0.91). IFN-α increased serum IL-6 (1826 ± 513%, t6 = −7.5, p < 0.001), IL-7 (39 ± 12%, t6 = −3.6, p = 0.01), IL-10 (328 ± 48%, t6 = −12.8, p < 0.001), and IFN-γ (272 ± 64%, t6 = −7.0, p < 0.001) at 4–6 h, and increased serum TNF-α (49 ± 7.6%, t6 = −7.5, p < 0.001), IL-8 (39 ± 12%, t6 = −3.5, p = 0.013), and C-reactive protein (1320 ± 459%, t6 = −7.2, p < 0.001) at 24 h. IFN-α induced temporary mood changes and sickness symptoms after 4–6 h, measured as an increase in POMS-2 total mood score, confusion and fatigue, and a decrease in vigor and friendliness (all p ≤ 0.04). No association was found between changes in peripheral inflammation and changes in PET or mood measures. Our work suggests that brain TSPO-PET signal is highly dependent of inflammation-induced changes in ligand binding to plasma proteins. This limits its usefulness as a sensitive marker of neuroinflammation and consequently, data interpretation. Thus, our results can be interpreted as showing either that [11C]PBR28 is not sensitive enough under these conditions, or that there is simply no microglial activation in this model.
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- art (ämneskategori)
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- Av författaren/redakt...
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Nettis, MA
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Veronese, M
-
Nikkheslat, N
-
Mariani, N
-
Lombardo, G
-
Sforzini, L
-
visa fler...
-
Enache, D
-
Harrison, NA
-
Turkheimer, FE
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Mondelli,
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Pariante, CM
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visa färre...
- Artiklar i publikationen
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Translational ps ...
- Av lärosätet
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Karolinska Institutet