Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:145951544" >
Comparison of Cytom...
-
Wagner-Drouet, E
(författare)
Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-γ ELISpot Assay after Hematopoietic Stem Cell Transplantation
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:prod.swepub.kib.ki.se:145951544
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:145951544URI
-
https://doi.org/10.3390/diagnostics11020312DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Teschner, D
(författare)
-
Wolschke, C
(författare)
-
Schafer-Eckart, K
(författare)
-
Gartner, J
(författare)
-
Mielke, SKarolinska Institutet
(författare)
-
Schreder, M
(författare)
-
Kobbe, G
(författare)
-
Hilgendorf, I
(författare)
-
Klein, S
(författare)
-
Verbeek, M
(författare)
-
Ditschkowski, M
(författare)
-
Koch, M
(författare)
-
Lindemann, M
(författare)
-
Schmidt, T
(författare)
-
Rascle, A
(författare)
-
Barabas, S
(författare)
-
Deml, L
(författare)
-
Wagner, R
(författare)
-
Wolff, D
(författare)
-
Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Diagnostics (Basel, Switzerland): MDPI AG11:22075-4418
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Wagner-Drouet, E
-
Teschner, D
-
Wolschke, C
-
Schafer-Eckart, ...
-
Gartner, J
-
Mielke, S
-
visa fler...
-
Schreder, M
-
Kobbe, G
-
Hilgendorf, I
-
Klein, S
-
Verbeek, M
-
Ditschkowski, M
-
Koch, M
-
Lindemann, M
-
Schmidt, T
-
Rascle, A
-
Barabas, S
-
Deml, L
-
Wagner, R
-
Wolff, D
-
visa färre...
- Artiklar i publikationen
-
Diagnostics (Bas ...
- Av lärosätet
-
Karolinska Institutet