Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:146104530" >
DNA-launched RNA re...
DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice
-
- Szurgot, I (författare)
- Karolinska Institutet
-
- Hanke, L (författare)
- Karolinska Institutet
-
- Sheward, DJ (författare)
- Karolinska Institutet
-
visa fler...
-
- Vidakovics, LP (författare)
- Karolinska Institutet
-
- Murrell, B (författare)
- Karolinska Institutet
-
- McInerney, GM (författare)
- Karolinska Institutet
-
Liljestrom, P (författare)
-
visa färre...
-
(creator_code:org_t)
- 2021-02-04
- 2021
- Engelska.
-
Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1, s. 3125-
- Relaterad länk:
-
https://www.nature.c...
-
visa fler...
-
http://kipublication...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The outbreak of the SARS-CoV-2 virus and its rapid spread into a global pandemic made the urgent development of scalable vaccines to prevent coronavirus disease (COVID-19) a global health and economic imperative. Here, we characterized and compared the immunogenicity of two alphavirus-based DNA-launched self-replicating (DREP) vaccine candidates encoding either SARS-CoV-2 spike glycoprotein (DREP-S) or a spike ectodomain trimer stabilized in prefusion conformation (DREP-Secto). We observed that the two DREP constructs were immunogenic in mice inducing both binding and neutralizing antibodies as well as T cell responses. Interestingly, the DREP coding for the unmodified spike turned out to be more potent vaccine candidate, eliciting high titers of SARS-CoV-2 specific IgG antibodies that were able to efficiently neutralize pseudotyped virus after a single immunization. In addition, both DREP constructs were able to efficiently prime responses that could be boosted with a heterologous spike protein immunization. These data provide important novel insights into SARS-CoV-2 vaccine design using a rapid response DNA vaccine platform. Moreover, they encourage the use of mixed vaccine modalities as a strategy to combat SARS-CoV-2.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas