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RT-qPCR assay for d...
RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
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Barsoe, S (författare)
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Ullman, K (författare)
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Leijon, M (författare)
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Hedlund, KO (författare)
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- Klingstrom, J (författare)
- Karolinska Institutet
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Krarup, LI (författare)
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Andresen, L (författare)
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Quaade, ML (författare)
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Hammer, AS (författare)
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(creator_code:org_t)
- 2021-05-26
- 2021
- Engelska.
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Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 16:5, s. e0252022-
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https://journals.plo...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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PloS one
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