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Keratinocytes Regul...
Keratinocytes Regulate the Threshold of Inflammation by Inhibiting T Cell Effector Functions
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Seiringer, P (författare)
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Eyerich, S (författare)
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- Eyerich, K (författare)
- Karolinska Institutet
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Dittlein, D (författare)
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Pilz, AC (författare)
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- Scala, E (författare)
- Karolinska Institutet
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Ring, J (författare)
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Behrendt, H (författare)
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Cavani, A (författare)
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Traidl-Hoffmann, C (författare)
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(creator_code:org_t)
- 2021-06-26
- 2021
- Engelska.
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Ingår i: Cells. - : MDPI AG. - 2073-4409. ; 10:7
- Relaterad länk:
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https://www.mdpi.com...
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http://kipublication...
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https://doi.org/10.3...
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Abstract
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- Whilst the importance of keratinocytes as a first-line defense has been widely investigated, little is known about their interactions with non-resident immune cells. In this study, the impact of human keratinocytes on T cell effector functions was analyzed in an antigen-specific in vitro model of allergic contact dermatitis (ACD) to nickel sulfate. Keratinocytes partially inhibited T cell proliferation and cytokine production. This effect was dependent on the keratinocyte/T cell ratio and was partially reversible by increasing the number of autologous dendritic cells. The inhibition of T cell proliferation by keratinocytes was independent of the T cell subtype and antigen presentation by different professional antigen-presenting cells. Autologous and heterologous keratinocytes showed comparable effects, while the fixation of keratinocytes with paraformaldehyde abrogated the immunosuppressive effect. The separation of keratinocytes and T cells by a transwell chamber, as well as a cell-free keratinocyte supernatant, inhibited T cell effector functions to the same amount as directly co-cultured keratinocytes, thus proving that soluble factor/s account for the observed suppressive effects. In conclusion, keratinocytes critically control the threshold of inflammatory processes in the skin by inhibiting T cell proliferation and cytokine production.
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Cells
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- Av författaren/redakt...
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Seiringer, P
-
Eyerich, S
-
Eyerich, K
-
Dittlein, D
-
Pilz, AC
-
Scala, E
-
visa fler...
-
Ring, J
-
Behrendt, H
-
Cavani, A
-
Traidl-Hoffmann, ...
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visa färre...
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Cells
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Karolinska Institutet