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Serum miR371 in tes...
Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
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Myklebust, MP (författare)
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- Thor, A (författare)
- Karolinska Institutet
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Rosenlund, B (författare)
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Gjengsto, P (författare)
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Karlsdottir, A (författare)
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Brydoy, M (författare)
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Bercea, BS (författare)
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Olsen, C (författare)
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Johnson, I (författare)
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Berg, MI (författare)
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Langberg, CW (författare)
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Andreassen, KE (författare)
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- Kjellman, A (författare)
- Karolinska Institutet
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Haugnes, HS (författare)
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Dahl, O (författare)
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(creator_code:org_t)
- 2021-08-02
- 2021
- Engelska.
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1, s. 15582-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- MicroRNA-371a-3p (miR371) has been suggested as a sensitive biomarker in testicular germ cell cancer (TGCC). We aimed to compare miR371 with the classical biomarkers α-fetoprotein (AFP) and β-human chorionic gonadotropin (hCGβ). Overall, 180 patients were prospectively enrolled in the study, with serum samples collected before and after orchiectomy. We compared the use of digital droplet PCR (RT-ddPCR) with the quantitative PCR used by others for detection of miR371. The novel RT-ddPCR protocol showed high performance in detection of miR371 in serum samples. In the study cohort, miR371 was measured using RT-ddPCR. MiR371 detected CS1 of the seminoma and the non-seminoma sub-types with a sensitivity of 87% and 89%, respectively. The total sensitivity was 89%. After orchiectomy, miR371 levels declined in 154 of 159 TGCC cases. The ratio of miR371 pre- and post-orchiectomy was 20.5 in CS1 compared to 6.5 in systemic disease. AFP and hCGβ had sensitivities of 52% and 51% in the non-seminomas. MiR371 is a sensitive marker that performs better than the classical markers in all sub-types and clinical stages. Especially for the seminomas CS1, the high sensitivity of miR371 in detecting TGCC cells may have clinical implications.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Myklebust, MP
-
Thor, A
-
Rosenlund, B
-
Gjengsto, P
-
Karlsdottir, A
-
Brydoy, M
-
visa fler...
-
Bercea, BS
-
Olsen, C
-
Johnson, I
-
Berg, MI
-
Langberg, CW
-
Andreassen, KE
-
Kjellman, A
-
Haugnes, HS
-
Dahl, O
-
visa färre...
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Scientific repor ...
- Av lärosätet
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Karolinska Institutet