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Role of the scaveng...
Role of the scavenger receptor MARCO in alveolar macrophage binding of unopsonized environmental particles
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Palecanda, A (författare)
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Paulauskis, J (författare)
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Al-Mutairi, E (författare)
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Imrich, A (författare)
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Qin, GZ (författare)
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Suzuki, H (författare)
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Kodama, T (författare)
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- Tryggvason, K (författare)
- Karolinska Institutet
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Koziel, H (författare)
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Kobzik, L (författare)
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(creator_code:org_t)
- 1999-05-03
- 1999
- Engelska.
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Ingår i: The Journal of experimental medicine. - : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 189:9, s. 1497-1506
- Relaterad länk:
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http://jem.rupress.o...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Alveolar macrophages (AMs) avidly bind and ingest unopsonized environmental particles and bacteria through scavenger-type receptors (SRs). AMs from mice with a genetic deletion of the major macrophage SR (types AI and AII; SR−/−) showed no decrease in particle binding compared with SR+/+ mice, suggesting that other SRs are involved. To identify these receptors, we generated a monoclonal antibody (mAb), PAL-1, that inhibits hamster AM binding of unopsonized particles (TiO2, Fe2O3, and latex beads; 66 ± 5, 77 ± 2, and 85 ± 2% inhibition, respectively, measured by flow cytometry). This antibody identifies a protein of ∼70 kD on the AM surface (immunoprecipitation) that is expressed by AMs and other macrophages in situ. A cDNA clone encoding the mAb PAL-1–reactive protein isolated by means of COS cell expression was found to be 84 and 77% homologous to mouse and human scavenger receptor MARCO mRNA, respectively. Transfection of COS cells with MARCO cDNA conferred mAb-inhibitable TiO2 binding. Hamster MARCO also mediates AM binding of unopsonized bacteria (67 ± 5 and 47 ± 4% inhibition of Escherichia coli and Staphylococcus aureus binding by mAb PAL-1). A polyclonal antibody to human MARCO identified the expected ∼70-kD band on Western blots of lysates of normal bronchoalveolar lavage (BAL) cells (>90% AMs) and showed strong immunolabeling of human AMs in BAL cytocentrifuge preparations and within lung tissue specimens. In normal mouse AMs, the anti-MARCO mAb ED31 also showed immunoreactivity and inhibited binding of unopsonized particles (e.g., TiO2 ∼40%) and bacteria. The novel function of binding unopsonized environmental dusts and pathogens suggests an important role for MARCO in the lungs' response to inhaled particles.
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- art (ämneskategori)
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Palecanda, A
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Paulauskis, J
-
Al-Mutairi, E
-
Imrich, A
-
Qin, GZ
-
Suzuki, H
-
visa fler...
-
Kodama, T
-
Tryggvason, K
-
Koziel, H
-
Kobzik, L
-
visa färre...
- Artiklar i publikationen
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The Journal of e ...
- Av lärosätet
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Karolinska Institutet