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A novel method usin...
A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors
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- Sollerbrant, K (författare)
- Karolinska Institutet
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Elmen, J (författare)
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Wahlestedt, C (författare)
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Acker, J (författare)
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Leblois-Prehaud, H (författare)
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Latta-Mahieu, M (författare)
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Yeh, P (författare)
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Perricaudet, M (författare)
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(creator_code:org_t)
- Microbiology Society, 2001
- 2001
- Engelska.
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Ingår i: The Journal of general virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 82:Pt 9, s. 2051-2060
- Relaterad länk:
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https://doi.org/10.1...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- The baculovirus Autographa californica multiple nucleopolyhedrosis virus causes non-productive infection in mammalian cells. Recombinant baculovirus therefore has the capability to transfer and express heterologous genes in these cells if a mammalian promoter governs the gene of interest. We have investigated the possibility of using baculovirus as a tool to produce recombinant adeno-associated virus (rAAV). AAV has become increasingly popular as a vector for gene therapy and functional genomics efforts, although its use is hampered by the lack of a simple and efficient vector production method. We show here that co-infection of mammalian producer cells with three viruses – a baculovirus containing the reporter gene flanked by AAV ITRs, a baculovirus expressing the AAV rep gene and a helper adenovirus expressing the AAV cap gene – produces infectious rAAV particles. This baculovirus-based chimeric vector method may in future improve large-scale rAAV vector preparations and circumvent present-day problems associated with rAAV production.
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