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Analysis of Na+,K+-...
Analysis of Na+,K+-ATPase motion and incorporation into the plasma membrane in response to G protein-coupled receptor signals in living cells
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- Bertorello, AM (författare)
- Karolinska Institutet
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Komarova, Y (författare)
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Smith, K (författare)
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- Leibiger, IB (författare)
- Karolinska Institutet
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Efendiev, R (författare)
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Pedemonte, CH (författare)
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Borisy, G (författare)
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Sznajder, JI (författare)
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(creator_code:org_t)
- American Society for Cell Biology (ASCB), 2003
- 2003
- Engelska.
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Ingår i: Molecular biology of the cell. - : American Society for Cell Biology (ASCB). - 1059-1524 .- 1939-4586. ; 14:3, s. 1149-1157
- Relaterad länk:
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https://europepmc.or...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- Dopamine (DA) increases Na+,K+-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na+,K+-ATPase molecules within the plasma membrane ( Ridge et al., 2002 ). Analysis of Na+,K+-ATPase motion was performed in real-time in alveolar cells stably expressing Na+,K+-ATPase molecules carrying a fluorescent tag (green fluorescent protein) in the α-subunit. The data demonstrate a distinct (random walk) pattern of basal movement of Na+,K+-ATPase–containing vesicles in nontreated cells. DA increased the directional movement (by 3.5 fold) of the vesicles and an increase in their velocity (by 25%) that consequently promoted the incorporation of vesicles into the plasma membrane. The movement of Na+,K+-ATPase–containing vesicles and incorporation into the plasma membrane were microtubule dependent, and disruption of this network perturbed vesicle motion toward the plasma membrane and prevented the increase in the Na+,K+-ATPase activity induced by DA. Thus, recruitment of new Na+,K+-ATPase molecules into the plasma membrane appears to be a major mechanism by which dopamine increases total cell Na+,K+-ATPase activity.
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