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Synaptotagmin III i...
Synaptotagmin III isoform is compartmentalized in pancreatic beta-cells and has a functional role in exocytosis
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Brown, H (författare)
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- Meister, B (författare)
- Karolinska Institutet
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Deeney, J (författare)
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Corkey, BE (författare)
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- Yang, SN (författare)
- Karolinska Institutet
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Larsson, O (författare)
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Rhodes, CJ (författare)
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Seino, S (författare)
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- Berggren, PO (författare)
- Karolinska Institutet
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Fried, G (författare)
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(creator_code:org_t)
- American Diabetes Association, 2000
- 2000
- Engelska.
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Ingår i: Diabetes. - : American Diabetes Association. - 0012-1797 .- 1939-327X. ; 49:3, s. 383-391
- Relaterad länk:
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http://diabetes.diab...
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http://kipublication...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Synaptotagmin is involved in Ca2+-regulated secretion and has been suggested to serve as a general Ca2+ sensor on the membrane of secretory vesicles in neuronal cells. Insulin exocytosis from the pancreatic beta-cell is an example of a Ca2+-dependent secretory process. Previous studies of pancreatic beta-cells were unable to show presence of synaptotagmin I. We now present biochemical and immunohistochemical data showing that synaptotagmin III is present in pancreatic beta-cells as well as in the insulin-secreting cell line HIT-T15 and in rat insulinoma. By subcellular fractionation, we found synaptotagmin III in high-density fractions together with insulin and secretogranin I, indicating colocalization of synaptotagmin III and insulin in secretory granules. We could also show that blockade of synaptotagmin III by a specific antibody inhibited Ca2+-induced changes in beta-cell membrane capacitance, suggesting that synaptotagmin III is part of the functional protein complex regulating beta-cell exocytosis. The synaptotagmin III antibody did not affect the activity of the voltage-gated L-type Ca2+-channel. These findings are compatible with the view that synaptotagmin III, because of its distinct localization in the pancreatic beta-cell, functionally modulates insulin exocytosis. This indicates that synaptotagmin may have a general role in the regulation of exocytosis not only in neuronal cells but also in endocrine cells.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Diabetes
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Till lärosätets databas
- Av författaren/redakt...
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Brown, H
-
Meister, B
-
Deeney, J
-
Corkey, BE
-
Yang, SN
-
Larsson, O
-
visa fler...
-
Rhodes, CJ
-
Seino, S
-
Berggren, PO
-
Fried, G
-
visa färre...
- Artiklar i publikationen
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Diabetes
- Av lärosätet
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Karolinska Institutet