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Neutrophil-derived ...
Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions
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- Soehnlein, O (författare)
- Karolinska Institutet
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Xie, X (författare)
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Ulbrich, H (författare)
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- Kenne, E (författare)
- Karolinska Institutet
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- Rotzius, P (författare)
- Karolinska Institutet
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Flodgaard, H (författare)
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- Eriksson, EE (författare)
- Karolinska Institutet
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- Lindbom, L (författare)
- Karolinska Institutet
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(creator_code:org_t)
- The American Association of Immunologists, 2005
- 2005
- Engelska.
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Ingår i: Journal of immunology (Baltimore, Md. : 1950). - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 174:10, s. 6399-6405
- Relaterad länk:
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http://www.jimmunol....
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http://kipublication...
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https://doi.org/10.4...
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Abstract
Ämnesord
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- In acute inflammation, infiltration of neutrophils often precedes a second phase of monocyte invasion, and data in the literature suggest that neutrophils may directly stimulate mobilization of monocytes via neutrophil granule proteins. In this study, we present a role for neutrophil-derived heparin-binding protein (HBP) in monocyte arrest on endothelium. Adhesion of neutrophils to bovine aorta endothelial cells (ECs) or HUVEC-triggered secretion of HBP and binding of the protein to the EC surface. Blockade of neutrophil adhesion by treatment with a mAb to CD18 greatly reduced accumulation of HBP. In a flow chamber model, immobilized recombinant HBP induced arrest of human monocytes or monocytic Mono Mac 6 (MM6) cells to activated EC or plates coated with recombinant adhesion molecules (E-selectin, P-selectin, VCAM-1). However, immobilized recombinant HBP did not influence arrest of neutrophils or lymphocytes. Treatment of MM6 cells with recombinant HBP evoked a rapid and clear-cut increase in cytosolic free Ca2+ that was found to be critical for the HBP-induced monocyte arrest inasmuch as pretreatment with the intracellular calcium chelating agent BAPTA-AM abolished the evoked increase in adhesion. Thus, secretion of a neutrophil granule protein, accumulating on the EC surface and promoting arrest of monocytes, could contribute to the recruitment of monocytes at inflammatory loci.
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- ref (ämneskategori)
- art (ämneskategori)
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