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A novel conserved R...
A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis
- Artikel/kapitelEngelska2002
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American Society for Cell Biology (ASCB),2002
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1955417
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1955417URI
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https://doi.org/10.1091/mbc.e02-03-0138DOI
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Språk:engelska
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Sammanfattning på:engelska
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Synthesis of the ribosomal subunits from pre-rRNA requires a large number of trans-acting proteins and small nucleolar ribonucleoprotein particles to execute base modifications, RNA cleavages, and structural rearrangements. We have characterized a novel protein, RNA-binding domain-1 (RBD-1), that is involved in ribosome biogenesis. This protein contains six consensus RNA-binding domains and is conserved as to sequence, domain organization, and cellular location from yeast to human. RBD-1 is essential in Caenorhabditis elegans. In the dipteran Chironomus tentans, RBD-1 (Ct-RBD-1) binds pre-rRNA in vitro and anti-Ct-RBD-1 antibodies repress pre-rRNA processing in vivo. Ct-RBD-1 is mainly located in the nucleolus in an RNA polymerase I transcription-dependent manner, but it is also present in discrete foci in the interchromatin and in the cytoplasm. In cytoplasmic extracts, 20–30% of Ct-RBD-1 is associated with ribosomes and, preferentially, with the 40S ribosomal subunit. Our data suggest that RBD-1 plays a role in structurally coordinating pre-rRNA during ribosome biogenesis and that this function is conserved in all eukaryotes.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bauren, G
(författare)
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Jin, SB
(författare)
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Tong, YG
(författare)
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Burglin, TRKarolinska Institutet
(författare)
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Hellman, U
(författare)
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Wieslander, L
(författare)
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Karolinska Institutet
(creator_code:org_t)
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Ingår i:Molecular biology of the cell: American Society for Cell Biology (ASCB)13:10, s. 3683-36951059-15241939-4586
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