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A hypoallergenic de...
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Kronqvist, M
(författare)
A hypoallergenic derivative of the major allergen of the dust mite Lepidoglyphus destructor, Lep d 2.6Cys, induces less IgE reactivity and cellular response in the skin than recombinant Lep d 2
- Artikel/kapitelEngelska2001
Förlag, utgivningsår, omfång ...
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2001-10-19
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S. Karger AG,2001
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1960343
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1960343URI
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https://doi.org/10.1159/000049493DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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<i>Background:</i> The major allergen of the dust mite<i> Lepidoglyphus destructor</i>, Lep d 2, has been produced as a recombinant allergen (rLep d 2) with IgE reactivity both in vivo and in vitro. A modified form of rLep d 2 (rLep d 2.6Cys) obtained by site-directed mutagenesis has been shown to have a reduced IgE reactivity in vitro. In this study we have compared the ability of rLep d 2 and rLep d 2.6Cys to elicit positive skin prick tests and cellular responses among<i> L. destructor</i>-sensitized subjects. <i>Methods:</i> Seventeen subjects were skin prick-tested with rLep d 2, rLep d 2.6Cys, histamine and negative controls and 17–20 h later skin biopsy specimens were taken from the skin prick-tested sites. The biopsy specimens were stained immunohistochemically for EG2+, CD3+, CD1a+, mast cell tryptase+, and IgE+ cells. Dermal cell infiltrates were judged in hematoxylin and eosin staining. Total IgE and allergen-specific IgE were determined by CAP-RAST. <i>Results:</i> Compared to rLep d 2, rLep d 2.6Cys induced significantly smaller and fewer skin prick test reactions (p < 0.001) and dermal cell infiltrates (p < 0.05). Further, rLep d 2.6Cys induced fewer EG2+ cells (p < 0.001) but more tryptase+ cells (p < 0.05) than rLep d 2. A positive RAST to rLep d 2 was obtained for 88.2% of the subjects, while only 35.2% displayed a positive RAST to rLep d 2.6Cys. <i>Conclusion:</i> This study demonstrates that rLep d 2.6Cys is less able to evoke IgE-mediated reactions and cellular responses, as measured both in skin and in serum, than rLep d 2. In the future this hypoallergenic derivative may be a promising candidate molecule for immunotherapy of <i>L. destructor</i>-allergic patients.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Johansson, E
(författare)
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Whitley, P
(författare)
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Olsson, S
(författare)
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Gafvelin, GKarolinska Institutet
(författare)
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Scheynius, AKarolinska Institutet
(författare)
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van Hage-Hamsten, MKarolinska Institutet
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:International archives of allergy and immunology: S. Karger AG126:1, s. 41-491018-24381423-0097
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