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Smear Microscopy fo...
Smear Microscopy for Diagnosis of Pulmonary Tuberculosis in Eastern Sudan
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Shuaib, YA (författare)
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Khalil, EAG (författare)
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Schaible, UE (författare)
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Wieler, LH (författare)
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Bakheit, MAM (författare)
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Mohamed-Noor, SE (författare)
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Abdalla, MA (författare)
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Homolka, S (författare)
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Andres, S (författare)
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Hillemann, D (författare)
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- Lonnroth, K (författare)
- Karolinska Institutet
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Richter, E (författare)
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Niemann, S (författare)
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Kranzer, K (författare)
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(creator_code:org_t)
- Hindawi Limited, 2018
- 2018
- Engelska.
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Ingår i: Tuberculosis research and treatment. - : Hindawi Limited. - 2090-150X .- 2090-1518. ; 2018, s. 8038137-
- Relaterad länk:
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https://doi.org/10.1...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- Background. In Sudan, tuberculosis diagnosis largely relies on clinical symptoms and smear microscopy as in many other low- and middle-income countries. The aim of this study was to investigate the positive predictive value of a positive sputum smear in patients investigated for pulmonary tuberculosis in Eastern Sudan. Methods. Two sputum samples from patients presenting with symptoms suggestive of tuberculosis were investigated using direct Ziehl-Neelsen (ZN) staining and light microscopy between June to October 2014 and January to July 2016. If one of the samples was smear positive, both samples were pooled, stored at −20°C, and sent to the National Reference Laboratory (NRL), Germany. Following decontamination, samples underwent repeat microscopy and culture. Culture negative/contaminated samples were investigated using polymerase chain reaction (PCR). Results. A total of 383 samples were investigated. Repeat microscopy categorized 123 (32.1%) as negative, among which 31 were culture positive. This increased to 80 when PCR and culture results were considered together. A total of 196 samples were culture positive, of which 171 (87.3%), 14 (7.1%), and 11 (5.6%) were M. tuberculosis, M. intracellulare, and mixed species. Overall, 15.6% (57/365) of the samples had no evidence of M. tuberculosis, resulting in a positive predictive value of 84.4%. Conclusions. There was a discordance between the results of smear microscopy performed at local laboratories in the Sudan and at the NRL, Germany; besides, a considerable number of samples had no evidence of M. tuberculosis. Improved quality control for smear microscopy and more specific diagnostics are crucial to avoid possible overtreatment.
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Shuaib, YA
-
Khalil, EAG
-
Schaible, UE
-
Wieler, LH
-
Bakheit, MAM
-
Mohamed-Noor, SE
-
visa fler...
-
Abdalla, MA
-
Homolka, S
-
Andres, S
-
Hillemann, D
-
Lonnroth, K
-
Richter, E
-
Niemann, S
-
Kranzer, K
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visa färre...
- Artiklar i publikationen
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Tuberculosis res ...
- Av lärosätet
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Karolinska Institutet