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MicroRNA control of...
MicroRNA control of the myogenic cell transcriptome and proteome: the role of miR-16
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Lim, S (författare)
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Lee, DE (författare)
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Morena da Silva, F (författare)
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Koopmans, PJ (författare)
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Vechetti, IJ (författare)
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- von Walden, F (författare)
- Karolinska Institutet
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Greene, NP (författare)
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Murach, KA (författare)
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(creator_code:org_t)
- 2023-03-27
- 2023
- Engelska.
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Ingår i: American journal of physiology. Cell physiology. - : American Physiological Society. - 1522-1563 .- 0363-6143. ; 324:5, s. C1101-C1109
- Relaterad länk:
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http://kipublication...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- microRNAs (miRs) control stem cell biology and fate. Ubiquitously expressed and conserved miR-16 was the first miR implicated in tumorigenesis. miR-16 is low in muscle during developmental hypertrophy and regeneration. It is enriched in proliferating myogenic progenitor cells but is repressed during differentiation. The induction of miR-16 blocks myoblast differentiation and myotube formation while knockdown enhances it. Despite a central role for miR-16 in myogenic cell biology, how it mediates its potent effects is incompletely defined. In this investigation, global transcriptomic and proteomic analyses after miR-16 knockdown in proliferating C2C12 myoblasts revealed how miR-16 influences myogenic cell fate. Eighteen hours after miR-16 inhibition, ribosomal protein gene expression levels were higher relative to control myoblasts and p53 pathway-related gene abundance was lower. At the protein level at this same timepoint, miR-16 knockdown globally upregulated TCA cycle proteins while downregulating RNA metabolism-related proteins. miR-16 inhibition induced specific proteins associated with myogenic differentiation such as ACTA2, EEF1A2, and OPA1. We extend prior work in hypertrophic muscle tissue and show that miR-16 is lower in mechanically overloaded muscle in vivo. Our data collectively point to how miR-16 is implicated in aspects of myogenic cell differentiation. A deeper understanding of the role of miR-16 in myogenic cells has consequences for muscle developmental growth, exercise-induced hypertrophy, and regenerative repair after injury, all of which involve myogenic progenitors.
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- art (ämneskategori)
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